In-vivo diagnostic method by near infrared radiation

• US 6,258,340 B1
• Filed: 03/06/2000
• Issued: 07/10/2001
• Est. Priority Date: 12/07/1994
• Status: Expired due to Term

• Current Assignee: VisEn Medical, Inc. (Revvity, Inc.)
• Original Assignee: Der Freien Universitat Berline, Institut fur Diagnostikforschung GmbH
• Inventors: Licha, Kai, Speck, Ulrich, Semmler, Wolfhard, Hilger, Christoph-Stephan, Riefke, Björn
• Primary Examiner(s): JONES, DAMERON LEVEST
• Application Number:
  US09/518,947
• Time in Patent Office: 491 Days
• Field of Search: 424/1.11, 424/9.1, 424/9.3, 424/9.4, 424/9.5, 424/9.6, 424/9.7, 424/9.8, 852/4, 548/100, 548/146, 548/215, 548/300.1, 548/400
• US Class Current: 424/9.6
• CPC Class Codes: A61K 49/0017 Fluorescence in vivo
• CPC Class Codes: A61K 49/0021 the fluorescent group being a small organic molecule
• CPC Class Codes: A61K 49/0032 Methine dyes, e.g. cyanine dyes
• CPC Class Codes: A61K 49/005 characterised by the carrier molecule carrying the fluorescent agent
• CPC Class Codes: C09B 23/086 more than five >CH- groups
• CPC Class Codes: C09B 69/105 containing a methine or polymethine dye

Abstract

This invention relates to a in-vivo diagnostic method based on near infrared ra ation (NIR radiation) that uses water-soluble dyes and their biomolecule adducts, each having specific photophysical and pharmaco-chemical properties, as a contrast medium for fluorescence and transillumination diagnostics in the NIR range, to new dyes and pharmaceuticals containing such dyes.

Claims

1. An in-vivo diagnostic near infrared radiation method comprising:

(a) administering a compound of the general formula I

2. A method according to claim 1, wherein B within the general formula I is selected from the group consisting of an amino acid, a peptide, CDR (complementarity determining region), an antigen, a hapten, an enzyme substrate, an enzyme cofactor, biotin, a carotinoid, a hormone, a neurohormone, a neurotransmitter, a growth factor, a lymphokin, a lectin, a toxin, a carbohydrate, an oligosaccharide, a polysaccharide, a dextran, an oligonucleotide and a receptor-bonding pharmaceutical.

3. A method according to claim 1 wherein W within the general formula I is selected from the group consisting of (i) a carboxy or sulfonic acid group;

(ii) a carboxyalkyl group;

(iii) an alkoxycarbonyl or alkoxyoxoalkyl group containing up to 12 carbon atoms,

(iv) a group of the general formula IIII