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Restriction enzyme mediated adapter

  • US 6,258,539 B1
  • Filed: 04/30/1999
  • Issued: 07/10/2001
  • Est. Priority Date: 08/17/1998
  • Status: Expired due to Term
First Claim
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1. A method of analyzing a polynucleotide, said method comprisingforming a restriction fragment from the polynucleotide, wherein the restriction fragment has a first and second terminus and at least one of the termini is generated by a restriction endonuclease, joining a first adapter to a terminus of the restriction fragment, whereby an adapter-modified restriction fragment is produced, and hybridizing a terminus probe to a single strand of the adapter-modified restriction fragment at a position including the terminus generated by the restriction endonuclease, wherein the terminus probe has a constant and a variable region.

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