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Quantitation of RNA transcripts using genomic DNA as the internal amplification competitor

  • US 6,258,543 B1
  • Filed: 09/17/1999
  • Issued: 07/10/2001
  • Est. Priority Date: 05/02/1996
  • Status: Expired due to Term
First Claim
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1. A method for quantitation of gene expression which comprises:

  • a) extracting nucleic acid from a biological specimen wherein said nucleic acid comprises mRNA transcripts and endogenous genomic DNA;

    b) designing complementary probe sequences specific for said mRNA transcripts and said endogenous genomic DNA, wherein said probe sequences discriminate between said endogenous geinomic DNA and said mRNA transcripts by sequence heterogeneity;

    c) hybridizing said complementary probe sequences to said mRNA transcripts and endogenous genomic DNA;

    d) directly detecting said endogenous genomic DNA and said mRNA transcripts; and

    e) quantitatively determining gene expression as expressed by the ratio of said mRNA transcripts to said endogenous genomic DNA.

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