Method and apparatus for inactivation of biological contaminants using endogenous alloxazine or isoalloxazine photosensitizers
First Claim
1. A method for decontamination of a fluid, by inactivation of microorganisms therein, sufficiently effective such that said fluid can be administered to a patient, said fluid also containing a component selected from the group consisting of biologically active protein, blood, and blood constituents, without destroying the biological activity of such component, said method comprising:
- (a) adding an effective, non-toxic amount of an endogenous alloxazine or isoalloxazine photosensitizer to said fluid;
(b) exposing the fluid of step (a) to photoradiation sufficient to activate the endogenous photosensitizer;
(c) allowing said activated endogenous photosensitizer to inactivate said microorganisms.
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Accused Products
Abstract
Methods and apparatuses are provided for inactivation of microorganisms in a fluid containing blood or blood products and comprising biologically active proteins. The method includes the steps of adding an effective, non-toxic amount of an endogenous photosensitizer to the fluid; exposing the fluid to photoradiation sufficient to activate the endogenous photosensitizer; and allowing the activated endogenous photosensitizer to interfere with nucleic acid present in microorganisms in the fluid so that the microorganisms are inactivated. Isoalloxazines and K- and L-vitamins are among the preferred photosensitizers. Systems and apparatuses are also provided for decontamination of such fluids using photosensitizers which include means for adding photosensitizer to the fluid; a photopermeable container for exposing the fluid to an amount of photoradiation sufficient to activate the photosensitizer; and a photoradiation source, preferably comprising a light guide, for providing sufficient photoradiation of a type and amount selected to activate the photosensitizer. Means for controlling photosensitizer concentration in the fluid and rate of fluid flow through the container, including computer processors may be included in such systems.
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Citations
18 Claims
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1. A method for decontamination of a fluid, by inactivation of microorganisms therein, sufficiently effective such that said fluid can be administered to a patient, said fluid also containing a component selected from the group consisting of biologically active protein, blood, and blood constituents, without destroying the biological activity of such component, said method comprising:
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(a) adding an effective, non-toxic amount of an endogenous alloxazine or isoalloxazine photosensitizer to said fluid;
(b) exposing the fluid of step (a) to photoradiation sufficient to activate the endogenous photosensitizer;
(c) allowing said activated endogenous photosensitizer to inactivate said microorganisms. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. An extracorporeal method for decontamination of a fluid, by inactivation of microorganisms therein, sufficiently effective such that said fluid can be administered to a patient, said fluid also containing a component selected from the group consisting of biologically active protein, blood, and blood constituents, without destroying the biological activity of such component, said method comprising:
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(a) adding to said fluid an effective, non-toxic amount of an endogenous alloxazine or isoalloxazine photosensitizer which inactivates microorganisms in the presence of albumin;
(b) exposing the fluid of step (a) to photoradiation sufficient to activate the endogenous photosensitizer; and
(c) allowing said activated endogenous photosensitizer to inactivate said microorganisms.
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Specification