Method and apparatus for inactivation of biological contaminants using endogenous alloxazine or isoalloxazine photosensitizers

• US 6,258,577 B1
• Filed: 07/21/1998
• Issued: 07/10/2001
• Est. Priority Date: 07/21/1998
• Status: Expired due to Term

• Current Assignee: Terumo BCT Biotechnologies, LLC (Terumo Corporation)
• Original Assignee: Gambro, Inc. (DaVita Incorporated)
• Inventors: Wood, Edward C. Jr., Corbin, Frank III, Goodrich, Raymond Paul Jr.
• Primary Examiner(s): Weber, Jon P.
• Application Number:
  US09/119,666
• Time in Patent Office: 1,085 Days
• Field of Search: 435/2, 435/173.3, 435/173.1
• US Class Current: 435/173.3
• CPC Class Codes: A61L 2/0011 using physical methods
• CPC Class Codes: A61L 2/0047 Ultraviolet radiation
• CPC Class Codes: A61L 2/0052 Visible light
• CPC Class Codes: A61L 2/0082 using chemical substances
• CPC Class Codes: A61L 2/08 Radiation
• CPC Class Codes: A61L 2/10 Ultraviolet radiation
• CPC Class Codes: A61L 2/23 Solid substances, e.g. granules, powders, blocks, tablets
• CPC Class Codes: A61L 2202/22 Blood or products thereof
• CPC Class Codes: A61M 1/3616 Batch-type treatment
• CPC Class Codes: A61M 1/36222 Details related to the interface between cassette and machine
• CPC Class Codes: A61M 1/36224 with sensing means or components thereof
• CPC Class Codes: A61M 1/362264 the filter being a blood filter
• CPC Class Codes: A61M 1/362266 Means for adding solutions or substances to the blood
• CPC Class Codes: A61M 1/3683 using photoactive agents
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: C12N 7/04 Inactivation or attenuation; Producing viral sub-units

Abstract

Methods and apparatuses are provided for inactivation of microorganisms in a fluid containing blood or blood products and comprising biologically active proteins. The method includes the steps of adding an effective, non-toxic amount of an endogenous photosensitizer to the fluid; exposing the fluid to photoradiation sufficient to activate the endogenous photosensitizer; and allowing the activated endogenous photosensitizer to interfere with nucleic acid present in microorganisms in the fluid so that the microorganisms are inactivated. Isoalloxazines and K- and L-vitamins are among the preferred photosensitizers. Systems and apparatuses are also provided for decontamination of such fluids using photosensitizers which include means for adding photosensitizer to the fluid; a photopermeable container for exposing the fluid to an amount of photoradiation sufficient to activate the photosensitizer; and a photoradiation source, preferably comprising a light guide, for providing sufficient photoradiation of a type and amount selected to activate the photosensitizer. Means for controlling photosensitizer concentration in the fluid and rate of fluid flow through the container, including computer processors may be included in such systems.

Claims

1. A method for decontamination of a fluid, by inactivation of microorganisms therein, sufficiently effective such that said fluid can be administered to a patient, said fluid also containing a component selected from the group consisting of biologically active protein, blood, and blood constituents, without destroying the biological activity of such component, said method comprising:

(a) adding an effective, non-toxic amount of an endogenous alloxazine or isoalloxazine photosensitizer to said fluid;

(b) exposing the fluid of step (a) to photoradiation sufficient to activate the endogenous photosensitizer;

(c) allowing said activated endogenous photosensitizer to inactivate said microorganisms.

2. The method of claim 1 wherein said endogenous photosensitizer is a nucleic-acid-targeted non-toxic, photo-activatable compound which does not produce toxic photolytic breakdown products.

3. The method of claim 1 wherein said endogenous photosensitizer is 7,8-dimethyl-10-ribityl isoalloxazine.

4. The method of claim 1 wherein said photoradiation is done using light in the visible spectrum.

5. The method of claim 1 wherein said photoradiation is done using light in the ultraviolet spectrum.

6. The method of claim 1 wherein said fluid of step (a) is flowed past a source of photoradiation at a rate and depth selected to ensure penetration of the photoradiation through the fluid and inactivation of the microorganisms.

7. The method of claim 1 wherein said fluid comprises blood constituents.

8. The method of claim 1 wherein said fluid is whole blood.

9. The method of claim 1 wherein said fluid is a separated blood product.

10. The method of claim 1 wherein said microorganisms are viruses.

11. The method of claim 1 wherein said endogenous photosensitizer is added to anticoagulant and said anticoagulant is added to said fluid.

12. The method of claim 1 wherein said photosensitizer is capable of inactivating microorganisms in the absence of oxygen.

13. The method of claim 1 wherein said photosensitizer is capable of inactivating microorganisms at pH about 7.4.

14. The method of claim 1 wherein said photosensitizer is capable of inactivating microorganisms in the presence of plasma, cells or blood components.

15. The method of claim 1 wherein said photosensitizer inactivates microorganisms present in human blood.

16. A fluid comprising biologically active protein, blood or blood constituents and inactivated microorganisms and endogenous photosensitizer or photoproduct thereof, made by the method of claim 1.

17. A blood product comprising inactivated microorganisms and endogenous photosensitizer or photoproduct thereof, made by the method of claim 1.

18. An extracorporeal method for decontamination of a fluid, by inactivation of microorganisms therein, sufficiently effective such that said fluid can be administered to a patient, said fluid also containing a component selected from the group consisting of biologically active protein, blood, and blood constituents, without destroying the biological activity of such component, said method comprising:

(a) adding to said fluid an effective, non-toxic amount of an endogenous alloxazine or isoalloxazine photosensitizer which inactivates microorganisms in the presence of albumin;

(b) exposing the fluid of step (a) to photoradiation sufficient to activate the endogenous photosensitizer; and

(c) allowing said activated endogenous photosensitizer to inactivate said microorganisms.