Gels for encapsulation of biological materials
First Claim
1. A method for encapsulating biological material, comprising the steps of:
- providing a mixture of the biological material in an aqueous macromer solution comprising macromer and a photoinitiating dye activatable by radiation having a wavelength between 320 nm and 900 nm, said biological material selected from the group consisting of mammalian tissue and mammalian cells, said macromer comprising a water-soluble biocompatible polymer having at least two ethylenically unsaturated sites, wherein the macromer is selected from the group consisting of poly(alkalene oxide) polyvinyl alcohol) poly(vinylpyrrolidone), poly(ethyloxazoline), poly(amino acids), polysaccharides, and proteins;
generating small geometric shapes of said mixture; and
polymerizing the macromer by exposing the geometric shapes to light radiation, wherein the radiation has a wavelength between 320 nm and 900 nm.
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Abstract
This invention provides novel methods for the formation of biocompatible membranes around biological materials using photopolymerization of water soluble molecules. The membranes can be used as a covering to encapsulate biological materials or biomedical devices, as a “glue” to cause more than one biological substance to adhere together, or as carriers for biologically active species. Several methods for forming these membranes are provided. Each of these methods utilizes a polymerization system containing water-soluble macromers, species which are at once polymers and macromolecules capable of further polymerization. The macromers are polymerized using a photoinitiator (such as a dye), optionally a cocatalyst, optionally an accelerator, and radiation in the form of visible or long wavelength UV light. The reaction occurs either by suspension polymerization or by interfacial polymerization. The polymer membrane can be formed directly on the surface of the biological material, or it can be formed on material which is already encapsulated.
182 Citations
58 Claims
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1. A method for encapsulating biological material, comprising the steps of:
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providing a mixture of the biological material in an aqueous macromer solution comprising macromer and a photoinitiating dye activatable by radiation having a wavelength between 320 nm and 900 nm, said biological material selected from the group consisting of mammalian tissue and mammalian cells, said macromer comprising a water-soluble biocompatible polymer having at least two ethylenically unsaturated sites, wherein the macromer is selected from the group consisting of poly(alkalene oxide) polyvinyl alcohol) poly(vinylpyrrolidone), poly(ethyloxazoline), poly(amino acids), polysaccharides, and proteins;
generating small geometric shapes of said mixture; and
polymerizing the macromer by exposing the geometric shapes to light radiation, wherein the radiation has a wavelength between 320 nm and 900 nm. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
where F1═ - CONH, COO or NHCOO
X═
H, CH3, C2H5, C6H5, Cl, Br, OH or CH2 COOHF2═
COO, CONH, O or C6H4,R═
CH2 or -alkyl-,n≦
5, andm≦
2.
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7. The method of claim 1, wherein the photoinitiator is any dye which absorbs light having frequency between 320 nm and 900 nm, can form free radicals, is at least partially water soluble, and is non-toxic to the biological material at the concentration used for polymerization.
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8. The method of claim 1, wherein the photoinitiator is selected from the group consisting of 2,2-dimethoxy-2-phenylacetophenone and 2-methoxy-2-phenylacetophenone.
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9. The method of claim 1, wherein the macromer solution further comprises a cocatalyst and the photoinitiator is selected from the group consisting of ethyl eosin, eosin Y, fluorescein, 2,2dimethoxy-2-phenylacetophenone, 2-methoxy-2-phenylacetophenone, camphorquinone, rose bengal, methylene blue, erythrosin, phloxime, thionine, riboflavin and methylene green.
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10. The method of claim 9, wherein the cocatalyst is a primary, secondary, tertiary or quaternary amine.
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11. The method of claim 10, wherein the cocatalyst is selected from the group consisting of triethanolamine, triethylamine, ethanolamine, N-methyl diethanolamine, N,N-dimethyl benzylamine, dibenzyl amine, N-benzyl ethanolamine, N-isopropyl benzylamine, tetramethyl ethylenediamine, potassium persulfate, tetramethyl ethylenediamine, lysine, ornithine, histidine and arginine.
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12. The method of claim 9, wherein the cocatalyst is potassium persulfate.
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13. The method of claim 1, wherein the radiation has a wavelength between 350 nm and 700 nm.
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14. The method of claim 1, wherein the cells are primary cells or established cell lines.
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15. The method of claim 1, wherein the biological material is selected from pancreatic islet cells, human foreskin fibroblasts, Chinese hamster ovary cells, beta cell insulomas, lymphoblastic leukemia cells, mouse 3T3 fibroblasts, dopamine secreting ventral mesencephalon cells, neuroblastoid cells, adrenal medulla cells, and T-cells.
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16. The method of claim 1, wherein the geometric shapes are formed by coextrusion of the aqueous macromer solution mixed with the biological material with a non-toxic, non-immunogenic, non-miscible substance capable of maintaining droplet formation.
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17. The method of claim 16, wherein the non-miscible substance is oil.
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18. The method of claim 17, wherein the oil is mineral oil.
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19. The method of claim 1, wherein the geometric shapes are formed by coextrusion of the aqueous macromer solution mixed with the biological material in air.
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20. The method of claim 1, wherein the geometric shapes are formed by agitation of the aqueous macromer solution mixed with the biological material with a non-toxic, non-immunogenic, non-miscible substance.
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21. The method of claim 20, wherein the non-miscible substance is oil.
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22. The method of claim 1, wherein the biological material is first encapsulated in a microcapsule.
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23. The method of claim 22, wherein the microcapsule is comprised of ionically coagulatable or thermally coagulatable polymers which are non-toxic to the encapsulated material.
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24. The method of claim 23, wherein the microcapsule is comprised of alginate.
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25. The method of claim 23, wherein the microcapsule is comprised of chitosan.
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26. The method of claim 23, wherein the microcapsule is comprised of agarose.
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27. The method of claim 23, wherein the microcapsule is comprised of gelatin.
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28. The method of claim 1, wherein the macromer solution further comprises an accelerator to accelerate the rate of polymerization.
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29. The method of claim 28, wherein the accelerator is a small molecule containing an allyl, vinyl or acrylate group.
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30. The method of claim 29, wherein the accelerator is selected from the group consisting of N-vinyl pyrolidinone, 2-vinyl pyridine, 1-vinyl imidazole, 9-vinyl carbazole, acrylic acid and 2-allyl-2-methyl-1,3-cyclopentane dione.
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31. The method of claim 30, wherein the accelerator is N-vinyl pyrolidinone.
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32. The method of claim 1, where at least one of the ethylenically unsaturated sites is a C—
- C double bond.
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33. The method of claim 1, wherein the geometric shape is selected from the group consisting of cylinders, slabs, discoidal shapes, globular shapes and spheres.
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34. A method for encapsulating biological material, comprising the steps of:
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coating the biological material with photoinitiator, said biological material selected from the group consisting of tissue and cells;
suspending the coated material in an aqueous macromer solution, said macromer comprising a water soluble polymer having at least two ethylenically unsaturated sites; and
irradiating the suspension with light, wherein the light has a wavelength between 320 nm and 900 nm. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58)
where F1═ - CONH, COO or NHCOO
X═
H, CH3, C2H5,C6H5, Cl, Br, OH or CA OOHF2═
COO, CONH, O or C6 H4,R═
CH2 or -alkyl-,n≦
5, andm≦
2.
-
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41. The method of claim 34, wherein the photoinitiator is any dye which absorbs light having frequency between 320 nm and 900 nm, can form free radicals, is at least partially water soluble, and is non-toxic to the biological material at the concentration used for polymerization.
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42. The method of claim 34, wherein the photoinitiator is selected from the group consisting of 2,2-dimethoxy-2-phenylacetophenone and 2-methoxy-2-phenylacetophenone.
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43. The method of claim 34, wherein the macromer solution further comprises a cocatalyst and the photoinitiator is selected from the group consisting of ethyl eosin, eosin Y, fluorescein, 2,2-dimethoxy-2-phenylacetophenone, 2-methoxy-2-phenylacetophenone, camphorquinone, rose bengal, methylene blue, erythrosin, phloxime, thionine, riboflavin and methylene green.
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44. The method of claim 43, wherein the cocatalyst is a primary, secondary, tertiary or quaternary amine.
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45. The method of claim 44, wherein the cocatalyst is selected from the group consisting of triethanolamine, triethylamine, ethanolamine, N-methyl diethanolamine, N,N-dimethyl benzylamine, dibenzyl amine, N-benzyl ethanolamine, N-isopropyl benzylamine, tetramethyl ethylenediamine, tetramethyl ethylenediamine, lysine, ornithine, histidine and arginine.
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46. The method of claim 43, wherein the cocatalyst is potassium persulfate.
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47. The method of claim 34, wherein the radiation has a wavelength between 350 nm and 700 nm.
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48. The method of claim 34, wherein the cells are primary cells or established cell lines.
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49. The method of claim 34, wherein the biological material is selected from pancreatic islet cells, human foreskin fibroblasts, Chinese hamster ovary cells, beta cell insulomas, lymphoblastic leukemia cells, mouse 3T3 fibroblasts, dopamine secreting ventral mesencephalon cells, neuroblastoid cells, adrenal medulla cells, and T-cells.
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50. The method of claim 34, wherein the biological material is first encapsulated in a microcapsule.
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51. The method of claim 50, wherein the microcapsule is comprised of ionically coagulatable or thermally coagulatable polymers which are non-toxic to the encapsulated material.
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52. The method of claim 51, wherein the microcapsule is comprised of a polymer selected from the group consisting of alginate, chitosan, agarose and gelatin.
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53. The method of claim 34, wherein the macromer solution further comprises an accelerator to accelerate the rate of polymerization.
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54. The method of claim 53, wherein the accelerator is a small molecule containing an allyl, vinyl or acrylate group.
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55. The method of claim 54, wherein the accelerator is selected from the group consisting of N-vinyl pyrolidinone, 2-vinyl pyridine, 1-vinyl imidazole, 9-vinyl carbazole, acrylic acid and 2-allyl-2-methyl-1,3-cyclopentane dione.
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56. The method of claim 55, wherein the accelerator is N-vinyl pyrolidinone.
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57. The method of claim 34, wherein the macromer is poly(ethylene glycol), the biological material is mammalian cells, and the accelerator is N-vinyl pyrrolidinone.
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58. The method of claim 34, wherein at least one of the ethylenically unsaturated sites is a C—
- C double bond.
Specification