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Process for microfabrication of an integrated PCR-CE device and products produced by the same

  • US 6,261,431 B1
  • Filed: 12/28/1998
  • Issued: 07/17/2001
  • Est. Priority Date: 12/28/1998
  • Status: Expired due to Term
First Claim
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1. An integrated microvolume device for performing polymerase chain reaction and electrophoresis, the device comprising:

  • at least one reaction chamber, the reaction chamber comprisingtwo planar members coupled together at at least one point, wherein one planar member has a thickness of up to about 1 mm and is thicker than the other planar member;

    a cavity disposed within the planar members;

    at least one aperture in communication with the cavity to permit the introduction and withdrawal of fluids therefrom;

    a thin film metal heater disposed within the cavity; and

    a thermocouple having a temperature-sensing junction disposed adjacent to the cavity;

    an electrophoresis capillary; and

    channels in fluid communication with the cavity and the electrophoresis capillary.

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