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Method of assaying for RNA: protein interactions

  • US 6,261,772 B1
  • Filed: 01/29/1999
  • Issued: 07/17/2001
  • Est. Priority Date: 01/30/1998
  • Status: Expired due to Fees
First Claim
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1. A method for screening a test compound for its ability to modulate a RNA:

  • protein interaction comprising;

    i) preparing a reporter construct comprising, in order, a Tat-responsive promoter operably linked to a sequence encoding a heterologous RNA binding site, and an indicator gene, ii) preparing an effector construct comprising a promoter operably linked to a nucleic acid sequence encoding a fusion protein comprising a Tat protein activation domain and a protein cognate of said heterologous RNA binding sites, iii) introducing said reporter construct and said effector construct into a vertebrate host cell and culturing said host cell in the presence and absence of said test compound, and iv) measuring the level of expression of said indicator gene in the presence and absence of said test compound, a difference in the level of expression of said indicator gene in the presence of said test compound, as compared to the level of expression of said indicator gene in the absence of said test compound, being indicative of a test compound that modulates interaction of said heterologous RNA binding site with said protein cognate.

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