Nucleic acid arrays
First Claim
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1. An array of oligonucleotides, the array comprising:
- a planar solid support having at least a first surface; and
a plurality of different oligonucleotides attached to the first surface of the solid support at a density exceeding 400 different oligonucleotides/cm2, wherein each of the different oligonucleotides is attached to the surface of the solid support in a different known location, and has a different determinable sequence.
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Abstract
A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group, is provided to the substrate to bind at the selected areas. The process is repeated using a variety of monomers such as amino acids until sequences of a desired length are obtained. Detection methods and apparatus are also disclosed.
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Citations
39 Claims
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1. An array of oligonucleotides, the array comprising:
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a planar solid support having at least a first surface; and
a plurality of different oligonucleotides attached to the first surface of the solid support at a density exceeding 400 different oligonucleotides/cm2, wherein each of the different oligonucleotides is attached to the surface of the solid support in a different known location, and has a different determinable sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 36)
providing a planar solid support, said solid support having a plurality of compounds immobilized on a surface thereof, said compounds having protecting groups coupled thereto;
deprotecting a first portion of said plurality of compounds on said surface and not a second portion of said plurality of compounds;
reacting said first portion of said plurality of compounds with a first component of said oligonucleotide;
deprotecting at least a third portion of said plurality of compounds on said surface, said third portion comprising a fraction of said first portion of said plurality of compounds;
reacting said at least third portion of said plurality of compounds with a second component of said oligonucleotide; and
optionally repeating said binary synthesis steps to produce said oligonucleotide array.
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36. The array of claim 1, wherein the plurality of different oligontucleotides have known sequences.
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15. An array of nucleic acids, the array comprising:
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a planar support having at least a first surface; and
a plurality of different nucleic acids attached to the first surface of the solid support at a density exceeding 400 different nucleic acids/cm2, wherein each of the different nucleic acids is attached to the surface of the solid support in a different known location, has a different determinable sequence, wherein the different nucleic acids in the different known locations are at least 10% pure. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 30, 31, 32, 33, 34, 35, 37)
providing a planar, solid support, said solid support having a plurality of compounds immobilized on a surface thereof, said compounds having protecting groups coupled thereto;
deprotecting a first portion of said plurality of compounds on said surface and not a second portion of said plurality of compounds;
reacting said first portion of said plurality of compounds with a first reactant;
deprotecting at least a third portion of said plurality of compounds on said surface, said third portion comprising a fraction of said first portion of said plurality of compounds;
reacting said at least third portion of said plurality of compounds with a second reactant; and
optionally repeating said binary synthesis steps to produce said nucleic acid array.
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27. The array of claim 15, wherein the nucleic acids are covalently attached to the support.
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30. The array of claim 15, wherein the different known locations are square in shape.
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31. The array of claim 15, wherein the substrate is glass.
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32. The array of claim 15, wherein the substrate is silicon dioxide.
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33. The array of claim 15, wherein the substrate is (poly)tetrafluoroethylene, (poly)vinylidenedifluoride, polystyrene or polycarbonate.
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34. The method of claim 15, wherein the substrate is optically transparent.
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35. The array of claim 15, where in the substrate is functionalized with groups that attach to the plurality of different nucleic acids.
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37. The array of claim 15, wherein the plurality of different nucleic acids have known sequences.
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28. An array of nucleic acids, the array comprising:
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a planar support having at least a first surface; and
a plurality of different nucleic acids attached to the first surface of the solid support at a density exceeding 10,000 different nucleic acids/cm2, wherein each of the different nucleic acids is attached to the surface of the solid support in a different known location, and has a different determinable sequence. - View Dependent Claims (38)
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29. An array of nucleic acids, the an-ay comprising:
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a planar support having at least a first surface; and
a plurality of different nucleic acids attached to the first surface of the solid support at a density exceeding 400 different nucleic acids/cm2, wherein each of the different nucleic acids is attached to the surface of the solid support in a different know location, has a different determinable sequence, wherein the surface and the support are made from different materials. - View Dependent Claims (39)
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Specification