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Methods for purifying viruses

  • US 6,261,823 B1
  • Filed: 12/11/1997
  • Issued: 07/17/2001
  • Est. Priority Date: 12/13/1996
  • Status: Expired due to Term
First Claim
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1. A method of purifying adenovirus from a virus preparation, comprising the successive steps of:

  • a) subjecting the virus preparation to anion-exchange chromatography, wherein the adenovirus is eluted from an anion-exchange chromatographic medium; and

    b) subjecting the eluate of step a) which contains the adenovirus to size exclusion chromatography, wherein the adenovirus is eluted from a size exclusion chromatographic medium.

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