Scanning microscope in which a sample is simultaneously and optically excited at various points
First Claim
1. An optical apparatus, wherein an expanded laser beam is divided into several partial beams by micro lenses arranged next to one another, wherein each partial beam is focused onto a focal point by a common objective lens to optically excite a sample, wherein fluorescent light emanating from the individual focal points of the sample is registered by a photo sensor arranged behind the objective lens as seen from the sample, and wherein each photon of the fluorescent light coming from the sample and being registered by the photo sensor is excited by at least two photons of the laser beam.
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Accused Products
Abstract
An optical apparatus, especially a scanning microscope (1), wherein an expanded laser beam (2) is divided into several partial beams (4) by micro lenses (5) arranged next to one another. Each partial beam (4) is focused onto a focal point (11) by a common objective lens (7) to optically excite a sample (8). Fluorescent light emanating from the individual focal points (11) of the sample (8) is registered by a photo sensor (13) arranged behind the objective lens (7) as seen from the sample (8). Each photon of the fluorescent light coming from the sample (8) and being registered by the photo sensor (13) is excited by at least two photons of the laser beam (2).
76 Citations
11 Claims
- 1. An optical apparatus, wherein an expanded laser beam is divided into several partial beams by micro lenses arranged next to one another, wherein each partial beam is focused onto a focal point by a common objective lens to optically excite a sample, wherein fluorescent light emanating from the individual focal points of the sample is registered by a photo sensor arranged behind the objective lens as seen from the sample, and wherein each photon of the fluorescent light coming from the sample and being registered by the photo sensor is excited by at least two photons of the laser beam.
Specification