Binding acceleration techniques for the detection of analytes
First Claim
Patent Images
1. A method of detecting a target analyte in a sample comprising:
- a) concentrating said target analyte in a detection chamber comprising a detection electrode comprising a self-assembled monolayer and a covalently attached capture ligand;
b) binding said target analyte to said capture ligand to form an assay complex, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and
c) detecting the presence of said ETM using said detection electrode.
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Abstract
The invention relates to compositions and methods useful in the acceleration of binding of target analytes to capture ligands on surfaces. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the target analyte, either directly or indirectly, to allow electronic detection of the ETM.
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Citations
29 Claims
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1. A method of detecting a target analyte in a sample comprising:
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a) concentrating said target analyte in a detection chamber comprising a detection electrode comprising a self-assembled monolayer and a covalently attached capture ligand;
b) binding said target analyte to said capture ligand to form an assay complex, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and
c) detecting the presence of said ETM using said detection electrode. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of detecting a target analyte in a sample comprising:
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a) flowing said sample past a detection electrode comprising a self-assembled monolayer and a covalently attached capture ligand under conditions that result in the formation of an assay complex comprising said target analyte and said capture ligand, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and
c) detecting the presence of said ETM using said detection electrode. - View Dependent Claims (14, 15, 16)
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17. A method of detecting a target nucleic acid sequence in a sample comprising:
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a) indirectly or directly hybridizing said target sequence to a capture probe covalently attached to a detection electrode comprising a self-assembled monolayer to form an assay complex, wherein said assay complex is formed in the presence of a hybridization accelerator, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and
b) detecting the presence of said ETM using said detection electrode. - View Dependent Claims (18, 19)
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20. A method of detecting a target analyte in a sample comprising:
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a) adding said sample to a detection electrode comprising a covalently attached capture ligand under conditions that result in the formation of an assay complex comprising said target analyte and said capture ligand, wherein said conditions include the presence of mixing particles, wherein said assay complex further comprises at least one electron transfer moiety (ETM);
b) detecting the presence of said ETM using said detection electrode.
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21. A method of detecting a target analyte in a sample comprising:
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a) concentrating said target analyte in a detection chamber comprising a detection electrode comprising a covalently attached capture ligand, wherein said concentrating is done by placing said sample in an electric field between at least a first electrode and at least a second electrode sufficient to cause electrophoretic transport of said sample to said detection electrode and said detection electrode is a porous electrode positioned between said first and said second electrodes;
b) binding said target analyte to said capture ligand to form an assay complex, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and
c) detecting the presence of said ETM using said detection electrode.
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22. A method of detecting a target analyte in a sample comprising:
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a) concentrating said target analyte in a detection chamber comprising a detection electrode comprising a covalently attached capture ligand;
b) binding said target analyte to said capture ligand to form an assay complex, wherein said assay complex further comprises at least one electron transfer moiety (ETM) and a label probe comprising said ETM, and said label probe is positively charged; and
c) detecting the presence of said ETM using said detection electrode.
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23. A method of detecting a target analyte in a sample comprising:
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a) concentrating said target analyte in a detection chamber comprising a detection electrode comprising a covalently attached capture ligand, wherein said concentrating comprises including at least one volume exclusion agent in said detection chamber;
b) binding said target analyte to said capture ligand to form an assay complex, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and
c) detecting the presence of said ETM using said detection electrode.
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24. A method of detecting a target analyte in a sample comprising:
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a) concentrating said target analyte in a detection chamber comprising a detection electrode comprising a covalently attached capture ligand, wherein said concentrating comprises precipitating said target analyte;
b) binding said target analyte to said capture ligand to form an assay complex, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and
c) detecting the presence of said ETM using said detection electrode.
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25. A method of detecting a target analyte in a sample comprising:
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a) concentrating said target analyte in a detection chamber comprising a detection electrode comprising a covalently attached capture ligand, wherein said concentrating comprises including at least two reagents that form two separable solution phases, such that said target analyte concentrates in one of said phases;
b) binding said target analyte to said capture ligand to form an assay complex, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and
c) detecting the presence of said ETM using said detection electrode.
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26. A method of detecting a target analyte in a sample comprising:
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a) concentrating said target analyte in a detection chamber comprising a detection electrode comprising a covalently attached capture ligand, wherein said concentrating comprises binding said target analyte t o a shuttle particle;
b) binding said target analyte to said capture ligand to form an assay complex, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and
c) detecting the presence of said ETM using said detection electrode.
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27. A method of detecting a target analyte in a sample comprising:
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a) flowing said sample past a detection electrode comprising a covalently attached capture ligand under conditions that result in the formation of an assay complex comprising said target analyte and said capture ligand, wherein said assay complex further comprises at least one electron transfer moiety (ETM), wherein the configuration of said detection electrode results in mixing of said sample; and
b) detecting the presence of said ETM using said detection electrode. - View Dependent Claims (28)
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29. A method of detecting a target analyte in a sample comprising:
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a) flowing said sample past a detection electrode comprising a covalently attached capture ligand under conditions that result in the formation of an assay complex comprising said target analyte and said capture ligand, wherein said assay complex further comprises at least one electron transfer moiety (ETM), wherein said detection electrode is porous and is positioned such that said sample flows through said electrode; and
b) detecting the presence of said ETM using said detection electrode.
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Specification