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Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces

  • US 6,265,186 B1
  • Filed: 12/10/1999
  • Issued: 07/24/2001
  • Est. Priority Date: 04/11/1997
  • Status: Expired due to Term
First Claim
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1. A yeast cell comprising at least two copies of a desired gene integrated into its chromosomes, wherein said chromosome(s) comprise at least two DNA domains each suitable for integration of one or more copies of said desired gene, which domains comprise diploid loci or multiple copies of a single locus and are non-ribosomal RNA encoding DNA domains, and wherein at least two of said non-ribosomal RNA encoding DNA domains have at least one copy of the said desired gene integrated.

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