Pox virus containing DNA encoding a cytokine and/or a tumor associated antigen

• US 6,265,189 B1
• Filed: 06/02/1995
• Issued: 07/24/2001
• Est. Priority Date: 01/07/1991
• Status: Expired due to Term

• Current Assignee: Virogenetics Corporation
• Original Assignee: Virogenetics Corporation
• Inventors: Tartaglia, James, Paoletti, Enzo, Cox, William I.
• Primary Examiner(s): Crouch, Deborah
• Application Number:
  US08/460,736
• Time in Patent Office: 2,244 Days
• Field of Search: 435/320.1, 435/69.5, 435/70.1, 435/70.3, 435/69.1, 424/232.1
• US Class Current: 435/70.1
• CPC Class Codes: A61K 2039/5254 avirulent or attenuated
• CPC Class Codes: A61K 2039/5256 expressing foreign proteins
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/0011 Cancer antigens
• CPC Class Codes: A61K 39/001106 Her-2/neu/ErbB2, Her-3/ErbB3 or Her 4/ErbB4
• CPC Class Codes: A61K 39/001138 Tumor necrosis factors [TNF] or CD70
• CPC Class Codes: A61K 39/001139 Colony stimulating factors [CSF]
• CPC Class Codes: A61K 39/00114 Interleukins [IL]
• CPC Class Codes: A61K 39/001141 Interferons [IFN]
• CPC Class Codes: A61K 39/001151 p53
• CPC Class Codes: A61K 39/001182 Carcinoembryonic antigen [CEA]
• CPC Class Codes: A61K 39/00119 Melanoma antigens
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61K 39/145 Orthomyxoviridae, e.g. influenza virus
• CPC Class Codes: A61K 39/17 Newcastle disease virus
• CPC Class Codes: A61K 39/205 Rhabdoviridae, e.g. rabies virus
• CPC Class Codes: A61K 39/275 Poxviridae, e.g. avipoxvirus
• CPC Class Codes: A61K 39/285 Vaccinia virus or variola virus
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/005 : from viruses
• CPC Class Codes: C07K 14/33 : from Clostridium (G)
• CPC Class Codes: C07K 14/4746 : p53
• CPC Class Codes: C07K 14/525 : Tumour necrosis factor [TNF]
• CPC Class Codes: C07K 14/535 : Granulocyte CSF; Granulocyte-macrophage CSF
• CPC Class Codes: C07K 14/5406 : IL-4
• CPC Class Codes: C07K 14/5434 : IL-12
• CPC Class Codes: C07K 14/55 : IL-2
• CPC Class Codes: C07K 14/57 : IFN-gamma
• CPC Class Codes: C07K 14/705 : Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C07K 14/70532 : B7 molecules, e.g. CD80, CD86
• CPC Class Codes: C07K 14/82 : Translation products from oncogenes
• CPC Class Codes: C07K 2319/00 : Fusion polypeptide
• CPC Class Codes: C12N 15/00 : Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00
• CPC Class Codes: C12N 15/86 : Viral vectors
• CPC Class Codes: C12N 2710/16122 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2710/16222 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2710/16622 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2710/16722 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2710/24022 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2710/24043 : viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 2710/24122 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2710/24143 : viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 2730/10122 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2740/13022 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2740/15022 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2740/16122 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2740/16222 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2750/14322 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2760/12022 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2760/16122 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2760/16134 : Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 2760/18122 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2760/18422 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2760/18722 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2760/20022 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2760/20122 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2760/20134 : Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 2770/20022 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2770/24022 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2770/24122 : New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 7/00 : Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00

Abstract

Attenuated recombinant viruses containing DNA coding for a cytokine and/or a tumor associated antigen, as well as methods and compositions employing the viruses, are disclosed and claimed. The recombinant viruses can be NYVAC or ALVAC recombinant viruses. The DNA can code for at least on of: human tumor necrosis factor; nuclear phosphoprotein p53, wildtype or mutant; human melanoma-associated antigen; IL-2; IFNγ; IL-4; GNCSF; IL-12; B7; erb-B-2 and carcinoembryonic antigen. The recombinant viruses and gene products therefrom are useful for cancer therapy.

Claims

1. A method for expressing a gene product in a cell cultured in vitro comprising introducing into the cell a recombinant vaccinia virus selected from the group consisting of vP1200, vP1101, vP1098, vP1239, vP1241, vP1237, vP1244, vP1243, vP1248, NYVAC+IFNγ

+IL-2, vP1250, vP1246, NYVAC+IL-12;

vP1230, vP1245, NYVAC+IFNγ

+B7, vP1234, vP1233, vP1100 and vP1096.

2. A recombinant poxvirus containing therein exogenous DNA coding for a cytokine, exogenous DNA coding for a tumor-associated antigen, or exogenous DNA coding for a cytokine and a tumor-associated antigen in a non-essential region of the poxvirus genome, wherein the poxvirus expresses the exogenous DNA and the poxvirus is a recombinant vaccinia virus wherein regions C7L-K1L, J2R, B13R+B14R, A56R, A26L, and I4L have been deleted therefrom, or wherein the open reading frames for the thymidine kinase gene, the hemorrhagic region, the A type inclusion body region, the hemagglutinin gene, the host range gene region, and the large subunit, ribonucleotide reductase, have been deleted therefrom.

3. The recombinant poxvirus of claim 2, wherein the recombinant vaccinia virus is NYVAC.

4. The recombinant poxvirus of claim 3, wherein the exogenous DNA codes for at least one of:

human tumor necrosis factor α

;

nuclear phosphoprotein p53, wildtype or mutant;

human melanoma-associated antigen;

IL-2;

IFN-γ

;

IL-4;

GMCSF;

IL-12;

B7;

erb-B-2 and carcinoembryonic antigen.

5. The recombinant poxvirus of claim 2, wherein the exogenous DNA codes for at least one of:

human tumor necrosis factor α

;

nuclear phosphoprotein p53, wildtype or mutant;

human melanoma-associated antigen;

IL-2;

IFN-γ

;

IL-4;

GMCSF;

IL-12;

B7;

erb-B-2 and carcinoembryonic antigen.

6. A recombinant poxvirus containing therein exogenous DNA coding for a cytokinie and/or a tumor associated antigen, wherein the poxvirus is selected from the group consisting of vP1200, vP1101, vP1098, vP1239, vP1241, vP1237, vP1244, vP1243, vP1248, NYVAC+IFNγ

+IL-2, vP1250, vP1246, NYVAC+IL-12, vP1230, vP1245, NYVAC+IFNγ

+IR7, vP1234, vP1233, vP1100 and vP1096.