Device for trapping and assaying C14 labelled carbon dioxide and method of use

• US 6,265,221 B1
• Filed: 06/07/1999
• Issued: 07/24/2001
• Est. Priority Date: 12/09/1996
• Status: Expired due to Term

• Current Assignee: Noster System AB (Scorpius SAS)
• Original Assignee: Noster System AB (Scorpius SAS)
• Inventors: Nilsson, Stefan
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Gakh, Yelena G.
• Application Number:
  US09/319,492
• Time in Patent Office: 778 Days
• Field of Search: 73/232.2, 422/58, 422/71, 422/80, 422/84, 422/85, 422/86, 422/88, 436/57, 436/58, 436/133, 436/169
• US Class Current: 436/133
• CPC Class Codes: G01N 31/221 for investigating pH value
• CPC Class Codes: G01N 33/004 CO or CO2
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath
• CPC Class Codes: Y02A 50/20 Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
• CPC Class Codes: Y10S 436/90 Breath testing
• CPC Class Codes: Y10T 436/204998 Inorganic carbon compounds

Abstract

A device for use in trapping and assaying ¹⁴CO₂ in air exhaled by a person to which a ¹⁴CO labelled compound has been administered, in particular ¹⁴CO labelled urea, comprises first and second substantially flat oblong duct forming elements joined to each other at their edges except for one of their short edges and forming a duct between them. The first duct forming element includes a gas permeable matrix element for absorption of CO₂. The device is provided with indicator means for visual detection of CO₂ absorption and a film element of low β-radiation absorptivity interposed or interposable between the matrix element and a β-radiation measuring instrument. Also disclosed is a corresponding method.

Claims

1. A device for use in trapping and assaying ¹⁴CO₂ in air exhaled by a person to which a ¹⁴C labelled compound has been administered, comprising first and second substantially flat oblong duct forming elements having long and short edges joined to each other at their respective edges except for one of their short edges and forming a duct between them, the first duct forming element including a gas permeable matrix element for absorption of CO₂, the device being provided with an indicator for detection of CO₂ absorption and a film element of low β

-radiation absorptivity interposed or interposable between the matrix element and a β

-radiation measuring instrument.

2. The device of claim 1, wherein the film element has a thickness of less than 0.5 mm.

3. The device of claim 2, wherein the matrix element comprises a matrix and an absorbent for carbon dioxide.

4. The device of claim 3, wherein the absorbant for carbon dioxide comprises a strong base.

5. The device of claim 4, wherein the base is selected from the group consisting of lithium hydroxide, sodium hydroxide, calcium hydroxide and their mixtures.

6. The device of claim 1 wherein the matrix element is of such design and has such carbon dioxide absorption capacity as to become saturated with carbon dioxide within from about one to two minutes by passage of the air exhaled by an adult person.

7. The device of claim 1, wherein the indicator comprises a pH indicator.

8. The device of claim 1 wherein the second duct forming element has a through opening disposed opposite to the matrix element covered by the film element.

9. The device of claim 8, comprising a flexible flap secured at a closed short end and having a length sufficient for covering, in a first position, the through opening of the second duct forming element and, in a second position, the matrix element of the first duct forming element.

10. The device of claim 1, wherein the film element has a form of a flexible flap secured at the closed short end and having a length sufficient for covering the matrix element of the first duct forming element.

11. The device of claim 1, wherein the indicator is comprised by the matrix element.

12. The device of claim 1, wherein the indicator is disposed separate from the matrix element.

13. The device of claim 12, wherein the indicator comprises a pH indicator disposed in a second matrix element for absorption of CO₂.

14. The device of claim 1, wherein the film forming element is provided with perforations.

16. The device of claim 5, wherein the film element has a thickness of less than 0.015 mm.

17. The device of claim 16, wherein the indicator comprises a pH indicator.

18. The device of claim 17, wherein the indicator comprises a pH indicator adapted to indicate a pH change above pH 10 by change of color.

19. The device of claim 1, wherein the film element has a thickness of less than 0.05 mm.

20. The device of claim 1, wherein the indicator comprises a pH indicator.

15. A method for trapping and assaying ¹⁴C exhaled by a person under investigation for a disease or condition characterised by increased enzymic activity in the gastro-intestinal tract leading to carbon dioxide formation from an enzymatically degradable agent administered to said person, comprising:

making said person exhale into an open short end of a device comprising first and second substantially flat oblong duct forming elements joined to each other at their respective edges except for said short end, the first duct forming element having an air-permeable matrix element covered by or coverable by a protective element with low β

-radiation absorptivity, the matrix element including a carbon dioxide absorbant, the device further comprising an indicator for visual detection of CO₂ uptake by change of colour;

observing the indicator to detect a colour change, and stopping exhalation into the device upon observation of such change;

optionally covering the matrix element with said protective element;

measuring β

-radiation originating from the matrix element for a period of time by bringing the device into contact with an apparatus for β

-radiation measurement; and

comparing the number of registered decay events with that obtained from healthy persons.