Purification process for anti-parasitic fermentation product

• US 6,265,571 B1
• Filed: 07/12/1999
• Issued: 07/24/2001
• Est. Priority Date: 07/12/1999
• Status: Expired due to Fees

• Current Assignee: Blue Ridge Pharmaceuticals, Inc. (IDEXX Laboratories Incorporated)
• Original Assignee: Magellan Laboratories, Inc. (Cardinal Health, Inc.), Blue Ridge Pharmaceuticals, Inc. (IDEXX Laboratories Incorporated)
• Inventors: Hill, John A., Shi, Qian
• Primary Examiner(s): Peselev, Elli
• Application Number:
  US09/351,751
• Time in Patent Office: 743 Days
• Field of Search: 536/7.1, 536/127
• US Class Current: 536/127
• CPC Class Codes: C07H 17/08 Hetero rings containing eight or more ring members, e.g. erythromycins

Abstract

A process for purifying the bacterial fermentation product ivermectin, particularly for use as a reference standard compound in a governmental regulatory approval process. The process includes the use of reversed-phase flash column chromatography coupled with gradient elution. An exemplary eluent mixture includes acetonitrile, methanol and water.

Claims

1. A process for purifying ivermectin, comprising:

(a) obtaining substantially impure ivermectin;

(b) loading the substantially impure ivermectin on a chromatographic column suitable for reversed-phase flash column chromatography;

(c) initiating reversed-phase flash chromatography by applying a first volume of an eluent mixture to the column, the eluent mixture comprising acetonitrile, lower alkyl alcohol and water;

(d) establishing an eluent gradient in the column by adding a series of subsequent volumes of the acetonitrile/lower alkyl alcohol/water eluent mixture to the column, wherein each of the first and the subsequent volumes of the eluent mixture has a ratio of acetonitrile to lower alkyl alcohol to water that varies according to a predetermined profile; and

(e) collecting predetermined fractions eluted from the column, the predetermined fractions comprising substantially purified ivermectin.

2. The process of claim 1, wherein the chromatographic column comprises a C₁₈ silica gel column.

3. The process of claim 1, wherein the predetermined profile of ratios of acetonitrile to lower alkyl alcohol to water in the first and the subsequent volumes of eluent mixture comprises a range from 0:

5;

5 to 5;

4;

1.

4. The process of claim 3, wherein the predetermined fractions collected in step (d) correspond to four respective volumes of eluent mixture wherein the acetonitrile, lower alkyl alcohol and water are present in the eluent mixture in the following four respective ratios 5:

2;

3, 5;

3;

2, 5;

3;

1 and 5;

4;

1.

5. The process of claim 3, wherein the predetermined fractions collected in step (d) correspond to two respective volumes of eluent mixture wherein the acetonitrile, lower alkyl alcohol and water are present in the eluent mixture in the following two respective ratios 5:

3;

2 and 5;

3;

1.

15. The process of any of claims 1, 6 or 11, wherein a purity level of the substantially purified ivermectin comprises at least about 98%.

6. A process for purifying ivermectin, comprising:

(a) obtaining substantially impure ivermectin;

(b) loading the substantially impure ivermectin on a chromatographic column suitable for reversed-phase flash column chromatography;

(c) initiating reversed-phase flash chromatography by applying a first volume of an eluent mixture to the column, the eluent mixture comprising acetonitrile, methanol and water;

(d) establishing an eluent gradient in the column by adding a series of subsequent volumes of the acetonitrile/methanol/water eluent mixture to the column, wherein each of the first and the subsequent volumes of the eluent mixture has a ratio of acetonitrile to methanol to water that varies according to a predetermined profile; and

(e) collecting predetermined fractions eluted from the column, the predetermined fractions comprising substantially purified ivermectin.

7. The process of claim 6, wherein the chromatographic column comprise a C₁₈ silica gel column.

8. The process of claim 6, wherein the predetermined profile of ratios of acetonitrile to methanol to water in the first and the subsequent volumes of eluent mixture comprises arrange from 0:

5;

5 to 5;

4;

1.

9. The process of claim 8, wherein the predetermined fractions collected in step (d) correspond to four respective volumes of eluent mixture wherein the acetonitrile, methanol and water are present in the eluent mixture in the following four respective ratios 5:

2;

3, 5;

3;

2, 5;

3;

1 and 5;

4;

1.

10. The process of claim 8, wherein the predetermined fractions collected in step (d) correspond to two respective volumes of eluent mixture wherein the acetonitrile, methanol and water are present in the eluent mixture in the following two respective ratios 5:

3;

2 and 5;

3;

1.

11. A process for purifying ivermectin, comprising:

(a) obtaining substantially impure ivermectin;

(b) loading the substantially impure ivermectin on a chromatographic column suitable for reversed-phase flash column chromatography;

(c) initiating reversed-phase flash chromatography by applying a first volume of an eluent mixture to the column, the eluent mixture comprising acetonitrile, methanol and water;

(d) establishing an eluent gradient in the column by adding a series of subsequent volumes of the acetonitrile/methanol/water eluent mixture to the column, wherein each of the first and in the subsequent volumes of the eluent mixture has a ratio of acetonitrile to methanol to water that varies according to a profile comprising a range from about 0;

5;

5 to about 5;

4;

1; and

(e) collecting predetermined fractions eluted from the column, the predetermined fractions comprising substantially purified ivermectin.

12. The process of claim 11, wherein the chromatographic column comprises a C₁₈ silica gel column.

13. A The process of claim 11, wherein the predetermined fractions collected in step (d) correspond to four respective volumes of eluent mixture wherein the acetonitrile, methanol and water are present in the eluent mixture in the following four respective ratios 5:

2;

3, 5;

3;

2, 5;

3;

1 and 5;

4;

1.

14. The process of claim 11, wherein the predetermined fractions collected in step (d) correspond to two respective volumes of eluent mixture wherein the acetonitrile, methanol and water are present in the eluent mixture in the following two respective ratios 5:

3;

2 and 5;

3;

1.