Proteoglycan-reduced soft tissue xenografts
First Claim
1. An article of manufacture comprising a substantially non-immunogenic, proteoglycan-reduced soft tissue xenograft for implantation into a human, produced bya. removing at least a portion of a soft tissue from a non-human animal to provide a xenograft;
- b. washing the xenograft in water and alcohol;
c. subjecting the xenograft to a cellular disruption treatment;
whereby the treated xenograft comprises substantially only dead cells and a plurality of proteoglycans; and
d. digesting the xenograft with a proteoglycan-depleting factor to remove substantially a plurality of proteoglycans from the xenograft, whereby the xenograft is substantially non-immunogenic and has substantially the same mechanical properties as the native soft tissue.
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Accused Products
Abstract
The invention provides an article of manufacture comprising a substantially non-immunogenic soft tissue xenograft for implantation into humans. The invention further provides methods for preparing a soft tissue xenograft by removing at least a portion of a soft tissue from a non-human animal to provide a xenograft; washing the xenograft in saline and alcohol; subjecting the xenograft to cellular disruption treatment; and digesting the xenograft with a proteoglycan-depleting factor and/or glycosidase and optionally following with a capping treatment. The invention also provides an article of manufacture produced by the above-identified method of the invention. The invention further provides a soft tissue xenograft for implantation into a human including a portion of a soft tissue from a non-human animal, wherein the portion has extracellular components and substantially only dead cells. The extracellular components have reduced proteoglycan molecules. Each of the xenografts of the invention are substantially non-immunogenic and have substantially the same mechanical properties as a corresponding native soft tissue.
150 Citations
31 Claims
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1. An article of manufacture comprising a substantially non-immunogenic, proteoglycan-reduced soft tissue xenograft for implantation into a human, produced by
a. removing at least a portion of a soft tissue from a non-human animal to provide a xenograft; -
b. washing the xenograft in water and alcohol;
c. subjecting the xenograft to a cellular disruption treatment;
whereby the treated xenograft comprises substantially only dead cells and a plurality of proteoglycans; and
d. digesting the xenograft with a proteoglycan-depleting factor to remove substantially a plurality of proteoglycans from the xenograft, whereby the xenograft is substantially non-immunogenic and has substantially the same mechanical properties as the native soft tissue. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A soft tissue xenograft for implantation into a human comprising
a portion of a soft tissue from a non-human animal, wherein the portion includes a plurality of substantially only dead cells and a plurality of extracellular components, the extracellular components having reduced proteoglycans, whereby the portion of the soft tissue is substantially non-immunogenic and has substantially the same mechanical properties as the native soft tissue.
Specification