Crystallization media

• US 6,267,935 B1
• Filed: 06/25/1999
• Issued: 07/31/2001
• Est. Priority Date: 06/26/1998
• Status: Expired due to Term

• Current Assignee: Washington University In St Louis
• Original Assignee: University of Washington
• Inventors: Hol, Wim G. J., Sarfaty, Steven H., Kim, Hidong, Stewart, Lansing J.
• Primary Examiner(s): POWELL, WILLIAM A
• Application Number:
  US09/344,007
• Time in Patent Office: 767 Days
• Field of Search: 117/206, 117/927, 422/245.1, 422/215, 422/256, 436/4, 436/15
• US Class Current: 422/245.1
• CPC Class Codes: B01L 3/06 Crystallising dishes
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C30B 29/58 Macromolecular compounds
• CPC Class Codes: C30B 7/00 Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions from molten solvents C30B9/00; by normal or gradient freezing C30B11/00; under a protective fluid C30B27/00

Abstract

In one aspect, the present invention provides crystallization solutions useful for crystallizing proteins and other molecules, especially macromolecules. In a presently preferred embodiment, the crystallization solutions of the invention are combined as four crystallization solution sets. Each of the four crystallization solution sets includes forty eight different crystallization solutions. Each individual crystallization solution includes a precipitant and a buffer, and optionally includes at least one additive. In another aspect, the present invention provides kits including a plurality of crystallization solutions of the present invention and at least one crystallization plate that preferably includes a plurality of reservoirs. Preferably the crystallization solutions are disposed within the reservoirs of the crystallization plates.

Claims

1. A set of crystallization solutions comprising a set selected from the group of sets consisting of Crystallization Solution Set I, Crystallization Solution Set II, Crystallization Solution Set III and Crystallization Solution Set IV.

2. A set of crystallization solutions of claim 1 comprising Crystallization Solution Set I.

3. A set of crystallization solutions of claim 2 consisting of Crystallization Solution Set I.

4. A set of crystallization solutions of claim 1 comprising Crystallization Solution Set II.

5. A set of crystallization solutions of claim 4 consisting of Crystallization Solution Set II.

6. A set of crystallization solutions of claim 1 comprising Crystallization Solution Set III.

7. A set of crystallization solutions of claim 6 consisting of Crystallization Solution Set III.

8. A set of crystallization solutions of claim 1 comprising Crystallization Solution Set IV.

9. A set of crystallization solutions of claim 8 consisting of Crystallization Solution Set IV.

10. A subset of crystallization solutions comprising a subset selected from the group of subsets consisting of solution numbers 1, 9, 10 and 28 of Crystallization Solution Set I, solution numbers 1, 8, 14, 26, 30 and 34 of Crystallization Solution Set II, solution numbers 2, 11, 18, 25, 26, 28, 29, 31, 38, 41 and 46 of Crystallization Solution Set III and solution numbers 2, 3, 5, 6, 7, 8, 17, 22, 24, 32, 34, 37, 43 and 48 of Crystallization Solution Set IV.

11. A subset of crystallization solutions of claim 10 comprising solution numbers 1, 9, 10 and 28 of Crystallization Solution Set I.

12. A subset of crystallization solutions of claim 10 comprising solution numbers 1, 8, 14, 26, 30 and 34 of Crystallization Solution Set II.

13. A subset of crystallization solutions of claim 10 comprising solution numbers 2, 11, 18, 25, 26, 28, 29, 31, 38, 41 and 46 of Crystallization Solution Set III.

14. A subset of crystallization solutions of claim 10 comprising solution numbers 2, 3, 5, 6, 7, 8, 17, 22, 24, 32, 34, 37, 43 and 48 of Crystallization Solution Set IV.

15. A kit comprising at least one crystallization plate and a set of crystallization solutions comprising a set selected from the group of sets consisting of Crystallization Solution Set I, Crystallization Solution Set II, Crystallization Solution Set III and Crystallization Solution Set IV.

16. The kit of claim 15 wherein the set of crystallization solutions comprises Crystallization Solution Set I.

17. The kit of claim 15 wherein the set of crystallization solutions comprises Crystallization Solution Set II.

18. The kit of claim 15 wherein the set of crystallization solutions comprises Crystallization Solution Set III.

19. The kit of claim 15 wherein the set of crystallization solutions comprises Crystallization Solution Set IV.

20. A kit comprising at least one crystallization plate and a subset of crystallization solutions comprising a subset selected from the group of subsets consisting of solution numbers 1, 9, 10 and 28 of Crystallization Solution Set I, solution numbers 1, 8, 14, 26, 30 and 34 of Crystallization Solution Set II, solution numbers 2, 11, 18, 25, 26, 28, 29, 31, 38, 41 and 46 of Crystallization Solution Set III and solution numbers 2, 3, 5, 6, 7, 8, 17, 22, 24, 32, 34, 37, 43 and 48 of Crystallization Solution Set IV.

21. The kit of claim 20 wherein the subset of crystallization solutions comprises solution numbers 1, 9, 10 and 28 of Crystallization Solution Set I.

22. The kit of claim 20 wherein the subset of crystallization solutions comprises solution numbers 1, 8, 14, 26, 30 and 34 of Crystallization Solution Set II.

23. The kit of claim 20 wherein the subset of crystallization solutions comprises solution numbers 2, 11, 18, 25, 26, 28, 29, 31, 38, 41 and 46 of Crystallization Solution Set III.

24. The kit of claim 20 wherein the subset of crystallization solutions comprises solution numbers 2, 3, 5, 6, 7, 8, 17, 22, 24, 32, 34, 37, 43 and 48 of Crystallization Solution Set IV.