Isoalloxazine derivatives to neutralize biological contaminants

• US 6,268,120 B1
• Filed: 10/19/1999
• Issued: 07/31/2001
• Est. Priority Date: 10/19/1999
• Status: Expired due to Term

• Current Assignee: Terumo BCT Biotechnologies, LLC (Terumo Corporation)
• Original Assignee: Gambro, Inc. (DaVita Incorporated)
• Inventors: Goodrich, Raymond Paul Jr., Platz, Matthew Stewart
• Primary Examiner(s): Shah, Mukund J.
• Assistant Examiner(s): Liu, Hong
• Application Number:
  US09/420,652
• Time in Patent Office: 651 Days
• Field of Search: 544/251, 514/251, 435/2
• US Class Current: 435/2
• CPC Class Codes: A61L 2/0076 using a photocatalyst or photosensitiser
• CPC Class Codes: A61L 2/0088 Liquid substances
• CPC Class Codes: A61L 2/10 Ultraviolet radiation
• CPC Class Codes: A61L 2202/22 Blood or products thereof
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: C07D 475/14 Benz [g] pteridines, e.g. riboflavin

Abstract

Methods are provided for neutralization of microorganisms in fluids or on surfaces. Preferably the fluids contain blood or blood products and comprise biologically active proteins. Preferred methods include the steps of adding an activation-effective amount of a microorganism neutralizer with an isoalloxazine backbone to a fluid and exposing the fluid to a triggering event. Preferred triggering events include light of a suitable wavelength and intensity to activate the microorganism neutralizer or a pH sufficient to activate the microorganism neutralizer. Other fluids, including juices, water and the like, may also be decontaminated by these methods as may surfaces of foods, animal carcasses, wounds, food preparation surfaces and bathing and washing vessel surfaces. Compounds with an isoalloxazine backbone are also provided.

Claims

1. A method for treating a fluid to neutralize microorganisms which may be present therein, said fluid containing one or more components selected from the group consisting of protein, blood, and blood constituents, said method comprising:

(a) adding to said fluid a neutralization-effective amount of a microorganism neutralizer of formula;

 

wherein X is a halogen selected from the group consisting of chlorine, bromine and iodine, R^a, R^b and R^c are, independently of each other, selected from the group consisting of hydrogen, optionally substituted hydrocarbyl, and halogen selected from the group consisting of chlorine, bromine and iodine, and n is an integer from 0 to 20;

(b) exposing the fluid of step (a) to a triggering event whereby said microorganisms are neutralized.

2. A method for treating a fluid to neutralize microorganisms which may be present therein, said fluid containing one or more components selected from the group consisting of protein, blood, and blood constituents, said method comprising:

(a) adding to said fluid a neutralization-effective amount of a microorganism neutralizer of formula;

 

wherein W is a water soluble group;

(b) exposing the fluid of step (a) to a triggering event whereby said microorganisms are neutralized.

3. A non-toxic composition comprising a blood product additive photosensitizer for inactivating microrganisms suitable for administration to a patient having the structure

wherein R is selected from the group consisting of hydrogen and optionally substituted straight chain or branched alkyl having from 1 to 20 carbon atoms.

4. A non-toxic composition comprising a blood product additive photosensitizer for inactivating microrganisms suitable for administration to a patient having the structure

wherein R is selected from the group consisting of hydrogen and optionally substituted straight chain or branched alkyl having from 1 to 20 carbon atoms.

5. A non-toxic composition comprising a blood product additive photosensitizer for inactivating microrganisms suitable for administration to a patient having the structure:

wherein W is a water soluble group.

6. A method of making a compound having structure:

wherein W is a water soluble group, comprising;

(a) photolyzing carboxyriboflavin;

(b) reacting (a) with oxallylchloride;

(c) reacting (b) with a member of the group consisting of ascorbate, glucosamine, protected glucose derivatives, diethylene glycol and triethylene glycol.

7. A method of making a compound having the structure:

where W is a water soluble group, comprising;

(a) contacting

 

with sodium azide;

(b) reacting (a) with

 

and POCl₃.

(c) reacting (b) with a water solubilizing group.