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Method for isolating and recovering target DNA or RNA molecules having a desired nucleotide sequence

  • US 6,268,133 B1
  • Filed: 06/24/1998
  • Issued: 07/31/2001
  • Est. Priority Date: 06/25/1997
  • Status: Expired due to Fees
First Claim
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1. A method for denaturing or separating double-stranded nucleic acid molecules, said method comprising contacting one or more double-stranded nucleic acid molecules with a denaturant selected from the group consisting of(a) one or more amino acids comprising:

  • glycine, α

    -alanine, isoleucine, leucine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine or valine, (b) imidazole, and (c) one or more amino acid denaturants plus imidazole, thereby forming single-stranded nucleic acid molecules.

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