Xyloglucan-specific alkaline xyloglucanase from bacillus
First Claim
1. An isolated polypeptide exhibiting xyloglucan-specific xyloglucanase activity, wherein the polypeptide is derived from a Bacillus species and exhibits at least 50% of the xyloglucanase activity at pH 7 relative to its xyloglucanase activity at the pH optimum for its activity.
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Abstract
A xyloglucanase having a relative xyloglucanase activity of at least 50% at pH 7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from eg Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from eg B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.
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Citations
12 Claims
- 1. An isolated polypeptide exhibiting xyloglucan-specific xyloglucanase activity, wherein the polypeptide is derived from a Bacillus species and exhibits at least 50% of the xyloglucanase activity at pH 7 relative to its xyloglucanase activity at the pH optimum for its activity.
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9. An isolated polypeptide selected from the group consisting of:
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(a) a polypeptide comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO;
4 and(b) a polypeptide having xyloglucan-specific xyloglucanase activity having an amino acid sequence that is at least 90% identical to amino acids 1-537 of SEQ ID NO;
4 as determined by GAP provided in the GCG program package, Version 8, with a GAP creation penalty of 3.0 and a GAP extension penalty of 0.1.- View Dependent Claims (10)
(i) an isolated and purified polypeptide as defined in claim 9 and (ii) one or more enzymes selected from the group consisting of proteases, β
-glucanases, hemicellulases, lipases, peroxidases, laccases, α
-amylases, glucoamylases, cutinases, pectinases, reductases, oxidases, phenoloxidases, ligninases, pullulanases, pectate lyases, xylanases, pectin acetyl esterases, polygalacturonases, rhamnogalacturonases, pectin lyases, other mannanases, pectin methylesterases, cellobiohydrolases, transglutaminases;
cellulases (endoglucanases) other than the polypeptide as defined in claim 9;
xyloglucanases other than the polypeptide as defined in claim 9; and
mixtures of any of the foregoing.
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11. An isolated polypeptide selected from the group consisting of
(a) a polypeptide comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO: - 2 and
(b) a polypeptide having xyloglucan-specific xyloglucanase activity having an amino acid sequence that is at least 90% identical to amino acids 30-261 of SEQ ID NO;
2 as determined by GAP provided in the GCG program package, Version 8, with a GAP creation penalty of 3.0 and a GAP extension penalty of 0.1.- View Dependent Claims (12)
(i) an isolated and purified polypeptide according to claim 11 and (ii) one or more enzymes selected from the group consisting of proteases, β
-glucanases, hemicellulases, lipases, peroxidases, laccases, α
-amylases, glucoamylases, cutinases, pectinases, reductases, oxidases, phenoloxidases, ligninases, pullulanases, pectate lyases, xylanases, pectin acetyl esterases, polygalacturonases, rhamnogalacturonases, pectin lyases, other mannanases, pectin methylesterases, cellobiohydrolases, transglutaminases;
cellulases (endoglucanases) other than the polypeptide as defined in claim 9;
xyloglucanases other than the polypeptide as defined in claim 9; and
mixtures of any of the foregoing.
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Specification