Xyloglucan-specific alkaline xyloglucanase from bacillus

• US 6,268,197 B1
• Filed: 07/07/1998
• Issued: 07/31/2001
• Est. Priority Date: 07/07/1997
• Status: Expired due to Fees

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novozymes A/S (Novozymes)
• Inventors: Bjornvad, Mads Eskelund, Outtrup, Helle, Schulein, Martin, Jorgensen, Per Lina
• Primary Examiner(s): Weber, Jon P.
• Application Number:
  US09/110,959
• Time in Patent Office: 1,120 Days
• Field of Search: 435/209, 435/263, 510/320, 510/392, 510/530
• US Class Current: 435/209
• CPC Class Codes: C11D 3/38636 containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
• CPC Class Codes: C12N 9/2434 acting on beta-1,4-glucosidic bonds

Abstract

A xyloglucanase having a relative xyloglucanase activity of at least 50% at pH 7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from eg Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from eg B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.

Claims

1. An isolated polypeptide exhibiting xyloglucan-specific xyloglucanase activity, wherein the polypeptide is derived from a Bacillus species and exhibits at least 50% of the xyloglucanase activity at pH 7 relative to its xyloglucanase activity at the pH optimum for its activity.

2. A polypeptide as defined in claim 1, wherein said Bacillus species is Bacillus licheniformis.

3. A polypeptide as defined in claim 2, wherein said Bacillus licheniformis is Bacillus licheniformis ATCC 14580.

4. A polypeptide as defined in claim 1, wherein said Bacillus species is Bacillus agaradhaerens.

5. A polypeptide as defmed in claim 4, wherein said Bacillus agaradhaerens is B. agaradhaerens, NCIMB 40482.

6. A detergent composition comprising the polypeptide as defined in claim 1 and a surfactant.

7. A process for machine treatment of a fabric, said process comprising treating the fabric during a washing cycle of a machine washing process with a washing solution comprising a polypeptide as defined in claim 1.

8. A method for treatment of cellulosic fibres, yarn, or woven or non-woven fabric, said method comprising treating the cellulosic fibres, yarn, woven or non-woven fabric during a scouring process step with a solution comprising a polypeptide as defined in claim 1.

9. An isolated polypeptide selected from the group consisting of:

(a) a polypeptide comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO;

4 and

(b) a polypeptide having xyloglucan-specific xyloglucanase activity having an amino acid sequence that is at least 90% identical to amino acids 1-537 of SEQ ID NO;

4 as determined by GAP provided in the GCG program package, Version 8, with a GAP creation penalty of 3.0 and a GAP extension penalty of 0.1.

10. An enzyme preparation comprising:

(i) an isolated and purified polypeptide as defined in claim 9 and

(ii) one or more enzymes selected from the group consisting of proteases, β

-glucanases, hemicellulases, lipases, peroxidases, laccases, α

-amylases, glucoamylases, cutinases, pectinases, reductases, oxidases, phenoloxidases, ligninases, pullulanases, pectate lyases, xylanases, pectin acetyl esterases, polygalacturonases, rhamnogalacturonases, pectin lyases, other mannanases, pectin methylesterases, cellobiohydrolases, transglutaminases;

cellulases (endoglucanases) other than the polypeptide as defined in claim 9;

xyloglucanases other than the polypeptide as defined in claim 9; and

mixtures of any of the foregoing.

11. An isolated polypeptide selected from the group consisting of

(a) a polypeptide comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:

2 and

(b) a polypeptide having xyloglucan-specific xyloglucanase activity having an amino acid sequence that is at least 90% identical to amino acids 30-261 of SEQ ID NO;

2 as determined by GAP provided in the GCG program package, Version 8, with a GAP creation penalty of 3.0 and a GAP extension penalty of 0.1.

12. An enzyme preparation comprising:

(i) an isolated and purified polypeptide according to claim 11 and

(ii) one or more enzymes selected from the group consisting of proteases, β

-glucanases, hemicellulases, lipases, peroxidases, laccases, α

-amylases, glucoamylases, cutinases, pectinases, reductases, oxidases, phenoloxidases, ligninases, pullulanases, pectate lyases, xylanases, pectin acetyl esterases, polygalacturonases, rhamnogalacturonases, pectin lyases, other mannanases, pectin methylesterases, cellobiohydrolases, transglutaminases;

cellulases (endoglucanases) other than the polypeptide as defined in claim 9;

xyloglucanases other than the polypeptide as defined in claim 9; and

mixtures of any of the foregoing.