Infectious clones of RNA viruses and vaccines and diagnostic assays derived thereof
First Claim
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1. An infectious clone based on a genome of a RNA virus comprising a PRRS virus, said infectious clone generated by a process comprising:
- providing a host cell not susceptible to infection by a wild-type of said RNA virus;
providing a recombinant nucleic acid based on the genome of said RNA virus, said recombinant nucleic acid comprising at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either;
transfecting said host cell with said recombinant nucleic acid; and
selecting for infectious clones.
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Abstract
An infectious clone based on the genome of a wild-type RNA virus is produced by the process of providing a host cell not susceptible to infection by the wild-type RNA virus, providing a recombinant nucleic acid based on the genome of the wild-type RNA virus, transfecting the host cell with the recombinant nucleic acid and selecting for infectious clones. The recombinant nucleic acid comprises at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either. The infectious clones can be used in single or dual purpose vaccines and in viral vector vaccines.
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8 Claims
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1. An infectious clone based on a genome of a RNA virus comprising a PRRS virus, said infectious clone generated by a process comprising:
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providing a host cell not susceptible to infection by a wild-type of said RNA virus;
providing a recombinant nucleic acid based on the genome of said RNA virus, said recombinant nucleic acid comprising at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either;
transfecting said host cell with said recombinant nucleic acid; and
selecting for infectious clones. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification