Purification of biologically active peptides from milk
First Claim
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1. A method of separating an exogenous component from a milk sample, comprising:
- a) subjecting the milk sample containing at least one of the following;
fat, casein micelles, somatic cells or particulate matter, to tangential flow filtration across a membrane of sufficient porosity to form a retentate and a permeate comprising the exogenous component;
b) subjecting the permeate to a chromatography capture device to remove the exogenous component and providing an effluent;
c) combining the effluent with the retentate; and
d) repeating Steps a) through c) at least once and until the exogenous component is free of fat, casein micelles, somatic cells or particulate matter, and wherein the recovery of the exogenous component is 75-90%.
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Abstract
A method of separating a soluble milk component from milk is disclosed. The method involves the use of tangential flow filtration across a membrane to form a retentate and a permeate, combining the permeate with the original milk sample, and repeating this procedure until the milk has been sufficiently purified. Preferably, the milk is combined with a chelating agent, such as EDTA, to improve the purification efficiency. This procedure is advantageously employed with milk from transgenic animals which have been genetically altered to express exogenous proteins, such as therapeutic proteins, in their milk.
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16 Claims
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1. A method of separating an exogenous component from a milk sample, comprising:
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a) subjecting the milk sample containing at least one of the following;
fat, casein micelles, somatic cells or particulate matter, to tangential flow filtration across a membrane of sufficient porosity to form a retentate and a permeate comprising the exogenous component;
b) subjecting the permeate to a chromatography capture device to remove the exogenous component and providing an effluent;
c) combining the effluent with the retentate; and
d) repeating Steps a) through c) at least once and until the exogenous component is free of fat, casein micelles, somatic cells or particulate matter, and wherein the recovery of the exogenous component is 75-90%. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification