Electrochemical methods for generation of a biological proton motive force and pyridine nucleotide cofactor regeneration
First Claim
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1. A method of promoting reductive processes in a biological system comprising the steps of:
- (a) providing an electrochemical bioreactor system having a cathode compartment equipped with a cathode and an anode compartment equipped with an anode, the cathode and anode compartments are separated by a cation selective membrane, wherein the cathode and anode are connected by a conductive material to a power supply; and
(b) placing a suitable amount of neutral red and a biological catalyst in the cathode compartment, wherein at least a portion of the neutral red is reduced, and wherein the biological catalyst comprises cells selected from the group consisting of Actinobacillus succinogenes and methanogenic archea.
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Abstract
Disclosed are methods using neutral red to mediate the interconversion of chemical and electrical energy. Electrically reduced neutral red has been found to promote cell growth and formation of reduced products by reversibly increasing the ratio of the reduced:oxidized forms of NAD(H) or NADP(H). Electrically reduced neutral red is able to serve as the sole source of reducing power for microbial cell growth. Neutral red is also able to promote conversion of chemical energy to electrical energy by facilitating the transfer of electrons from microbial reducing power to a fuel cell cathode.
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Citations
6 Claims
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1. A method of promoting reductive processes in a biological system comprising the steps of:
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(a) providing an electrochemical bioreactor system having a cathode compartment equipped with a cathode and an anode compartment equipped with an anode, the cathode and anode compartments are separated by a cation selective membrane, wherein the cathode and anode are connected by a conductive material to a power supply; and
(b) placing a suitable amount of neutral red and a biological catalyst in the cathode compartment, wherein at least a portion of the neutral red is reduced, and wherein the biological catalyst comprises cells selected from the group consisting of Actinobacillus succinogenes and methanogenic archea.
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2. A method of promoting reductive processes in a biological system comprising the steps of:
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(a) providing an electrochemical bioreactor system having a cathode compartment equipped with a cathode and an anode compartment equipped with an anode, the cathode and anode compartments are separated by a cation selective membrane, wherein the cathode and anode are connected by a conductive material to a power supply; and
(b) placing a suitable amount of neutral red and a biological catalyst in the cathode compartment, wherein at least a portion of the neutral red is reduced, wherein the biological catalyst comprises cells and wherein the cells comprise methanogens. - View Dependent Claims (3, 4)
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5. A method of promoting reductive processes in a biological system comprising the steps of:
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(a) providing an electrochemical bioreactor system having a cathode compartment equipped with a cathode and an anode compartment equipped with an anode, the cathode and anode compartments are separated by a cation selective membrane, wherein the cathode and anode are connected by a conductive material to a power supply; and
(b) placing a suitable amount of neutral red and a biological catalyst in the cathode compartment, wherein at least a portion of the neutral red is reduced, wherein the biological catalyst comprises cells and wherein the cells comprise succinogenic bacteria, and wherein the bacteria reduces fumarate to produce succinate.
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6. A method of promoting reductive processes in a biological system comprising the steps of:
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(a) providing an electrochemical bioreactor system having a cathode compartment equipped with a cathode and an anode compartment equipped with an anode, the cathode and anode compartments are separated by a cation selective membrane, wherein the cathode and anode are connected by a conductive material to a power supply; and
(b) placing a suitable amount of neutral red and a biological catalyst in the cathode compartment, wherein at least a portion of the neutral red is reduced, wherein the biological catalyst comprises an isolated enzyme that uses cells NADH or NADPH as a cofactor, wherein the cathode compartment comprises NAD (H) or NADP (H) wand an oxidized substrate for the enzyme and wherein the reduced neutral red transfers electrons to NAD+ or NADP+, and wherein the enzyme comprises an alcohol dehydrogenase, the oxidized substrate comprises an aldehyde or ketone, and a reduced product is produced, wherein the product comprises an alcohol.
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Specification