Device for incubating and monitoring multiwell assays

• US 6,271,022 B1
• Filed: 03/26/1999
• Issued: 08/07/2001
• Est. Priority Date: 03/12/1999
• Status: Expired due to Term

• Current Assignee: Biolog, Inc.
• Original Assignee: Biolog, Inc.
• Inventors: Bochner, Barry
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/277,353
• Time in Patent Office: 865 Days
• Field of Search: 422/63, 422/65, 422/68.1, 422/50, 422/52, 422/82.05, 422/82.08, 422/82.09, 435/287.3, 435/288.7, 356/51, 356/302, 356/346, 356/388, 356/395, 356/244, 382/128, 382/133
• US Class Current: 435/287.3
• CPC Class Codes: C12M 41/14 Incubators; Climatic chambers per se B01L1/00
• CPC Class Codes: G01N 2035/00306 Housings, cabinets, control panels (details)
• CPC Class Codes: G01N 2035/00356 Holding samples at elevated temperature (incubation)
• CPC Class Codes: G01N 2035/0425 Stacks, magazines or elevators for plates
• CPC Class Codes: G01N 2035/0498 Drawers used as storage or dispensing means for vessels or cuvettes
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates

Abstract

The present invention provides easy-to-use, adaptable, and convenient solutions to a heretofore unmet need for instruments that can incubate and kinetically monitor assays, especially multiwell assays. In particular, the present invention finds use in monitoring assays contained within various test formats, including, but not limited to microtiter plates, miniaturized test panels (e.g., MICROCARDS™), and petri plates.

Claims

1. An instrument comprising:

1) an enclosure cabinet;

     2) a plurality of sliding shelves mounted in said cabinet, wherein said shelves are capable of holding one or more assay plates;

     3) an imaging device mounted in said cabinet for simultaneously capturing within a field of view one or more images of one or more of said assay plates located on one or more of said shelves; and

     4) a means for moving said imaging device, whereby said imaging device can be selectively positioned, without moving said assay plates on said shelves, to simultaneously capture images of all wells in said field of view of said assay plates located on any of said shelves.

2. The instrument of claim 1, wherein said sliding shelves are capable of sliding into the field of view of said imaging device.

3. The instrument of claim 1, wherein said imaging device comprises a camera.

4. The instrument of claim 1, wherein said imaging device comprises a scanner.

5. The instrument of claim 1, wherein said cabinet is a temperature controlled incubator.

6. The instrument of claim 1, wherein said cabinet is gas-tight and can maintain a controlled level of a gas.

7. The intent of claim 1, wherein each of said shelves has a dimension of approximately eight by eight inches.

8. The instrument of claim 1, wherein said plurality of sliding shelves consists of at least twenty sliding shelves.

9. The instrument of claim 1, wherein one or more of said shelves are capable of holding at least two assay plates.

10. The instrument of claim 9, wherein said shelves contain one or more openings for viewing at least one multiwell assay plate from the bottom of said shelves.

11. The instrument of claim 9, wherein said shelves are capable of holding one or more assay plates selected from the group consisting of microplates, MICROCARDS™

, and petri dishes.

12. The instrument of claim 1, wherein said cabinet comprises an access door.

13. The instrument of claim 12, wherein said shelves are accessible from said door.

14. The instrument of claim 3, wherein said camera is selected from the group consisting of a CCD camera and a CMOS camera.

15. The instrument of claim 1, wherein said means for moving said imaging device comprises a motor.

16. The instrument of claim 15, wherein said motor is a stepper motor.

17. The instrument of claim 1, further comprising a means for controlling said instrument.

18. The instrument of claim 17, where said means for controlling said instrument controls elements of said instrument selected from the group consisting of:

internal temperature of said cabinet, internal lighting of said cabinet, internal gas composition of said cabinet, positions of said plurality of sliding shelves, position of said imaging device, activity of said means for moving said imaging device, imaging by said imaging device, door closing, door opening, and combinations thereof.

19. The instrument of claim 18, wherein said means for controlling said instrument comprises a computer.

20. The instrument of claim 19, wherein said computer is connected to said instrument by a standard RS-232 interface.

21. The instrument of claim 19, wherein said computer comprises electronics for receiving digital image information from said imaging device.

22. The instrument of claim 19, where said computer monitors elements of said instrument selected from the group consisting of:

internal temperature of said cabinet, internal lighting of said cabinet, internal gas composition of said cabinet, positions of said plurality of sliding shelves, position of said imaging device, activity of said means for moving said imaging device, imaging by said imaging device, door closing, door opening, and combinations thereof.

23. The instrument of claim 19, where said computer records data on elements of said instrument selected from the group consisting of:

internal temperature of said cabinet, internal lighting of said cabinet, internal gas composition of said cabinet, positions of said plurality of sliding shelves, position of said imaging device, activity of said means for moving said imaging device, imaging by said imaging device, door closing, door opening, and combinations thereof.

24. The instrument of claim 19, where said computer controls elements of said instrument selected from the group consisting of:

internal temperature of said cabinet, internal lighting of said cabinet, internal gas composition of said cabinet, positions of said plurality of sliding shelves, position of said imaging device, activity of said means for moving said imaging device, imaging by said imaging device, door closing, door opening, and combinations thereof.

25. The instrument of claim 19, wherein said computer records data on the images of the assay plates.

26. An instrument comprising:

1) an enclosure cabinet;

     2) a plurality of sliding shelves mounted in said cabinet, wherein said shelves are capable of holding two or more assay plates; and

     3) an imaging device mounted in said cabinet for capturing one or more images of said two or more assay plates located on each of said shelves, wherein each of said images captures all of said assay plates on any one or more of said shelves.

27. The instrument of claim 26, wherein said sliding shelves are capable of sliding into a field of view of said imaging device.

28. The instrument of claim 26, further comprising 4) a means for moving said imaging device, whereby said imaging device can be selectively positioned, without moving said assay plates on said shelves, to simultaneously capture images of all wells in a field of view of said assay plates located on any of said shelves.

29. A system comprising:

1) an enclosure cabinet;

     2) a plurality of sliding shelves mounted in said cabinet;

     3) two or more assay plates located on at least one of said sliding shelves; and

     4) an imaging device mounted in said cabinet for simultaneously capturing within a field of view one or more images of said two or more assay plates.

30. The system of claim 29, further comprising 5) a means for moving said imaging device, whereby said imaging device can be selectively positioned, without moving said assay plates on said shelves, to simultaneously capture images of all wells in said field of view of said assay plates located on any of said shelves.

31. A method for kinetically monitoring assay plates, comprising:

a) providing the system of claim 29; and

b) capturing one or more images of said two or more assay plates using said imaging device, at two or more separate time points.

32. The method of claim 31, wherein said system further comprises a means for moving said imaging device, whereby said imaging device can be selectively positioned, without moving said assay plates on said shelves, to simultaneously capture images of all wells in said field of view of said assay plates located on any of said shelves.