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Method of sequencing a nucleic acid

  • US 6,274,320 B1
  • Filed: 09/16/1999
  • Issued: 08/14/2001
  • Est. Priority Date: 09/16/1999
  • Status: Expired due to Term
First Claim
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1. A method for sequencing a nucleic acid, the method comprising:

  • providing one or more or more nucleic acid anchor primers linked to a solid support, wherein said solid support includes at least one optical fiber;

    providing a plurality of single-stranded circular nucleic acid templates;

    annealing an effective amount of the nucleic acid anchor primer to at least one of the single-stranded circular templates to yield a primed anchor primer-circular template complex;

    combining the primed anchor primer-circular template complex with a polymerase to generate an extended anchor primer covalently linked to a nucleic acid comprising multiple copies of a sequence complementary to the circular nucleic acid template;

    annealing an effective amount of a sequencing primer to one or more copies of said covalently linked complementary nucleic acid;

    extending the sequencing primer with a polymerase and a predetermined nucleotide triphosphate to yield a sequencing product and, if the predetermined nucleotide triphosphate is incorporated onto the 3′

    end of said sequencing primer, a sequencing reaction byproduct; and

    identifying the sequencing reaction byproduct, thereby determining the sequence of the nucleic acid.

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