Method and system for performing infrared study on a biological sample

• US 6,274,871 B1
• Filed: 10/22/1998
• Issued: 08/14/2001
• Est. Priority Date: 10/22/1998
• Status: Expired due to Fees

• Current Assignee: Vysis, Inc. (Abbott Laboratories Incorporated)
• Original Assignee: Vysis, Inc. (Abbott Laboratories Incorporated), The Procter & Gamble Company
• Inventors: Dukor, Rina K., Marcott, Curtis A.
• Primary Examiner(s): Hannaher, Constanine
• Assistant Examiner(s): Gabor, Otilia
• Application Number:
  US09/176,792
• Time in Patent Office: 1,027 Days
• Field of Search: 250/339.8, 250/339.2, 250/339.12
• US Class Current: 250/339.08
• CPC Class Codes: G01J 3/45 Interferometric spectrometry
• CPC Class Codes: G01N 2021/3595 using FTIR
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence
• CPC Class Codes: G02B 21/0004 specially adapted for specific applications

Abstract

A method and system performs Fourier transform infrared (FT-IR) imaging microspectroscopy on a biological sample fixed on a substrate with a supporting surface that generally reflects infrared light while generally transmitting visible light. Infrared light impinging on the biological sample is reflected by the supporting surface of the substrate. Infrared light from the sample is focused onto a focal-plane array detector with multiple pixels for detecting infrared images of the sample. The detected infrared images are processed to generate spectral images of the sample. The same biological sample is suitable for conventional pathological studies.

Claims

1. A method of examining biological samples comprising the steps of:

positioning a biological sample supported on a supporting surface at an imaging position, the supporting surface generally reflecting infrared light while generally transmitting visible light;

directing an infrared beam in the mid-infrared range to the biological sample;

collecting infrared images of the biological sample in a reflection-absorption mode by detecting infrared light reflected by the supporting surface and through the biological sample with a detector array having multiple detection pixels; and

deriving a Fourier transform infrared (FT-IR) spectrum from the collected infrared images.

2. A method as in claim 1, wherein the supporting surface includes a coating that is generally reflective of infrared light while generally transmitting visible light.

3. A method as in claim 1, further including visually selecting a portion of interest of the biological sample and positioning the selected portion for collecting infrared images thereof.

4. A method as in claim 1, wherein the biological sample includes cellular types that are cancerous.

5. A method as in claim 1, wherein the biological sample is a tissue sample.

6. A method as in claim 5, wherein the tissue sample is stained.

7. A method as in claim 1, wherein the biological sample is a blood sample.

8. A method as in claim 1, wherein the sample includes exfoliated cells.

9. A method as in claim 1, wherein the biological sample is selected from the group of a Papanicolau smear, a cervical specimen, an ectocervical specimen, an endocervical specimen, a vaginal specimen, and a uterus specimen.

10. A method of identifying cellular types in biological samples comprising the step of:

positioning a biological sample supported on a supporting surface at an imaging position, the supporting surface being generally reflective of infrared light while generally transmitting visible light;

inspecting the biological sample using visible light to identify a site of interest in the sample;

positioning the site of interest for infrared imaging with a Fourier transform infrared (FT-IR) imaging device, the FT-IR imaging device including an array detector having multiple detection pixels;

collecting infrared images of the site of interest in a reflection-absorption mode by directing an infrared beam in the mid-infrared range to the site of interest and detecting infrared light reflected by the supporting surface and through the biological sample from the site of interest; and

deriving an infrared spectrum from the collected infrared images for identifying cellular types at the site of interest.

11. A method as in claim 10, wherein the supporting surface includes a coatng that generally transmits visible light and generally reflects infrared light.

12. A method as in claim 10, wherein the cellular types are cancerous cells.

13. A method as in claim 10, wherein the inspecting step includes identifying cellular types at the site of interest based on visual appearance of the site of interest.

14. A method as in claim 10, wherein the biological sample is a tissue siaple.

15. A method as in claim 10, wherein the tissue sample is stained.

16. A method as in claim 10, wherein the biological sample includes exfoliated cells.

17. A method as in claim 10, wherein the biological sample is selected the group of a Papanicolau smear, a cervical specimen, an ectocervical specimen, an endocervical specimen, a vaginal specimen, and a uterus specimen.

18. A system for studying biological samples comprising:

a biological sample supported on a supporting surface generally reflecting infrared light and generally transmitting visible light;

a Fourier transform infrared (FT-IR) spectrometer for generating an input infrared beam in the mid-infrared range for illuminating the biological sample;

a focal-plane array detector having multiple detection pixels for detecting infrared images of the biological sample for derivation of spectral images of the sample; and

infrared optical elements for imaging the sample in a reflection-absorption mode wherein the infrared optical elements focuses infrared light reflected by the supporting surface and through the biological sample to the focal-plane array detector.

19. A system as in claim 18, further including optical elements for visually inspecting the biological sample for identifying a site of interest and a stage for positioning the site of interest for infrared image detection by the focal-plane array detector.

20. A system as in claim 18, wherein the supporting surface includes a coating that generally transmits visible light and generally reflects infrared light.

21. A method of examining biological samples comprising the steps of:

positioning a biological sample supported on a supporting surface at an imaging position, the supporting surface generally reflecting infrared light;

directing an infrared beam in the mid-infrared range to the biological sample;

collecting infrared images of the biological sample in a reflection-absorption mode by detecting infrared light reflected by the supporting surface and through the biological sample with a detector array having multiple detection pixels; and

deriving a Fourier transform infrared (FT-IR) spectrum from the collected infrared images.

22. A method as in claim 21, wherein the supporting surface includes a coating that is generally reflective of infrared light while generally transmitting visible light.

23. A method as in claim 22, further including visually selecting a portion of interest of the biological sample and positioning the selected portion for collecting infrared images thereof.

24. A method as in claim 21, wherein the biological sample includes cellular types that are cancerous.

25. A method as in claim 21, wherein the biological sample is a tissue sample.

26. A method as in claim 25, wherein the tissue sample is stained.

27. A method as in claim 21, wherein the biological sample is a blood sample.

28. A method as in claim 21, wherein the sample includes exfoliated cells.

29. A method as in claim 21, wherein the biological sample is selected from the group of a Papanicolau smear, a cervical specimen, an ectocervical specimen, an endocervical specimen, a vaginal specimen, and a uterus specimen.