Process and device for carrying out quantitative, fluorescence affinity tests
First Claim
1. Device for carrying out quantitative fluorescence-marked affinity tests by means of evanescent field excitation, having at least one light source (7, 7′
- ) which emits almost monochromatic light and directs light beams, having a wavelength which causes fluorescence of a marking substance bound to a chemical or biochemical partner of a general receptor/ligand system, at an angle α
to be defined by a predeterminable penetration depth d for the evanescent field onto an interface (20) of an optically transparent baseplate (1) made of a material whose refractive index n1 is greater than the refractive index n2 of the material above the interface (20), characterized in that the light is directed through the baseplate (1) onto the interface (20) of a reception region (2) which is in the form of a cuvette and has a thickness of between 0.001 and 0.5 mm, a sample container (10) for receiving the sample is arranged in such a way that a link is formed via a first possible connection (9), which is at least partially an opening between the reception region (2) in the form of a cuvette and the sample container (10) and a second possible connection (11) is linked to the reception region (2) in the form of a cuvette, the reception region (2) being covered on the opposite side from the baseplate (1) with a cover plate (3), and a detector (5) for picking up the fluorescent light being arranged on the same side of the baseplate (1) as the light source (7, 7′
).
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Accused Products
Abstract
A process and device are disclosed for carrying out in particular quantitative fluorescence immunity tests by means of evanescent field excitation, possibly on the basis of various known biochemical assays of systems generally composed of receptor-ligands. However, antibody-antigen systems are preferably evaluated. The invention should enable quantitative fluorescence immunity tests with various known biochemical assays to be carried out with a very simple device. For that purpose, a light source is used which emits rays of an almost monochromatic light having a wavelength which causes a marking substance bound to the antibody to become fluorescent. The light rays are directed at an angle α determined by a depth of penetration d previously determined for the evanescent field onto a boundary surface between an optically transparent base plate made of a material with a refraction index n1 is higher than the refraction index n2 of the material above the boundary surface and a cuvette-shaped sample-receiving area. The sample receiving area is covered at the side opposite to the base plate by a covering plate and a detector for sensing fluorescent light is arranged at the same side of the base plate as the light source.
45 Citations
29 Claims
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1. Device for carrying out quantitative fluorescence-marked affinity tests by means of evanescent field excitation, having at least one light source (7, 7′
- ) which emits almost monochromatic light and directs light beams, having a wavelength which causes fluorescence of a marking substance bound to a chemical or biochemical partner of a general receptor/ligand system, at an angle α
to be defined by a predeterminable penetration depth d for the evanescent field onto an interface (20) of an optically transparent baseplate (1) made of a material whose refractive index n1 is greater than the refractive index n2 of the material above the interface (20), characterized in that the light is directed through the baseplate (1) onto the interface (20) of a reception region (2) which is in the form of a cuvette and has a thickness of between 0.001 and 0.5 mm, a sample container (10) for receiving the sample is arranged in such a way that a link is formed via a first possible connection (9), which is at least partially an opening between the reception region (2) in the form of a cuvette and the sample container (10) and a second possible connection (11) is linked to the reception region (2) in the form of a cuvette, the reception region (2) being covered on the opposite side from the baseplate (1) with a cover plate (3), and a detector (5) for picking up the fluorescent light being arranged on the same side of the baseplate (1) as the light source (7, 7′
). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- ) which emits almost monochromatic light and directs light beams, having a wavelength which causes fluorescence of a marking substance bound to a chemical or biochemical partner of a general receptor/ligand system, at an angle α
- 24. Method for carrying out fluorescence immuno tests by means of evanescent field excitation, characterized in that a sample volume is taken from a sample container (10) through a reception region (2) in the form of a cuvette by suction, pressure or capillary forces and the marked chemical or biochemical components which are to be determined according to the biochemical assay are bound to the corresponding complementary chemical or biochemical components which are fixed on the surface in the reception region (2), and the fluorescent light is measured with a detector (5) by evanescent field excitation by means of the light source(s) (7, 7′
Specification