Methods for solid-phase nucleic acid amplification and sequencing
First Claim
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1. A method for the solid-phase amplification of nucleic acids, which comprises contacting a support comprising a polyfunctionalized solid support;
- a linker having a multiplicity of functional groups capable of binding to said solid support; and
an oligonucleotide primer bound to said linker wherein said primer is immobilized on said solid support via said linker by means of at least one covalent bond between said functional groups and said solid support;
with a sample comprising at least one nucleic acid complementary to the primer bound to the solid support in the presence of a polymerase, and under reaction conditions which effect elongation of said primer.
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Abstract
Methods for amplifying and sequencing nucleic acids are disclosed. In addition, supports for the solid phase amplification or sequencing of nucleic acids are disclosed. The supports comprise a solid support, a linker arm having functional groups capable of binding to the solid support, and an oligonucleotide bound to the linker arm.
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Citations
13 Claims
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1. A method for the solid-phase amplification of nucleic acids, which comprises contacting a support comprising a polyfunctionalized solid support;
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a linker having a multiplicity of functional groups capable of binding to said solid support; and
an oligonucleotide primer bound to said linker wherein said primer is immobilized on said solid support via said linker by means of at least one covalent bond between said functional groups and said solid support;
with a sample comprising at least one nucleic acid complementary to the primer bound to the solid support in the presence of a polymerase, and under reaction conditions which effect elongation of said primer. - View Dependent Claims (2, 8, 11, 12, 13)
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3. A method for the solid-phase amplification of nucleic acids, which comprises contacting a support comprising a polyfunctionalized solid support;
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a linker having a multiplicity of functional groups capable of binding to said solid support; and
a first oligonucleotide primer bound to said linker, wherein said primer is immobilized on said solid support via said linker by means of at least one covalent bond between said functional groups and said solid support;
with, in solution comprising at least one, nucleic acid complementary to the primer bound to the solid support in the presence of a polymerase, and a second amplification primer, under reaction conditions which effect elongation of said primer bound to the solid support and said second amplification primer, and amplification of said nucleic acid. - View Dependent Claims (4, 5, 6, 7, 9)
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10. A method for the solid-phase sequencing of nucleic acids, which comprises contacting a support comprising:
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a poly solid support;
a linker having a multiplicity of functional groups capable of binding to said solid support; and
an oligonucleotide primer bound to said linker wherein said primer is immobilized on said solid support by means of at least one covalent bond between said primer and said solid support;
with a sample comprising at least one nucleic acid complementary the primer bound to said support in the presence of suitable reagents, including a polymerase, and under appropriate reaction conditions for sequencing at least one nucleic acid sequence.
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Specification