Colorimetric sensor employing polydiacetylene membrane

• US 6,277,652 B1
• Filed: 12/23/1998
• Issued: 08/21/2001
• Est. Priority Date: 12/26/1997
• Status: Expired due to Fees

• Current Assignee: HOGY Medical Company Limited
• Original Assignee: HOGY Medical Company Limited
• Inventors: Takada, Kouichi, Inoue, Toshiki, Jo, Yoshio
• Primary Examiner(s): Chin, Christopher L.
• Assistant Examiner(s): Do, Pensee T.
• Application Number:
  US09/220,389
• Time in Patent Office: 972 Days
• Field of Search: 436/164, 436/512, 436/518, 436/805, 436/829, 436/905, 435/7.1, 435/287.1, 435/287.2, 435/287.9, 435/808, 435/969, 530/300, 424/143.1
• US Class Current: 436/518
• CPC Class Codes: C12Q 1/6804 Nucleic acid analysis using immunogens immunoassay G01N33/53
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: G01N 33/5432 Liposomes or microcapsules
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/969 Multiple layering of reactants
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/829 Liposomes, e.g. encapsulation
• CPC Class Codes: Y10S 436/905 Photochemical activation of reactions
• CPC Class Codes: Y10S 977/80 Nucleic acid, e.g. DNA or RNA

Abstract

Disclosed is a colorimetric sensor comprising polydiacetylene membrane liposomes, a polydiacetylene membrane film or fine particles coated with a polydiacetylene membrane, in which the polydiacetylene membrane is incorporated with a protein having a reduced molecular weight low enough not to cause color change in the polydiacetylene membrane. The examples of the reduced-molecular-weight proteins include an antibody Fab′ fragment, an antigenic protein of molecular weight of 100,000 or less, and a peptide consisting of 3-20 amino acid residue, which undergo an antigen-antibody reaction with an antigen or antibody contained in a sample. As the reduced-molecular-weight protein is also employed a combination of single-stranded DNA of 100 bases or less which hybridizes with single-stranded DNA contained in a sample to form a double-stranded DNA, and an antibody which reacts with said double-stranded DNA but does not react with the single-stranded DNA contained in the sample. A method for analysis of biosample is also disclosed, which comprises contacting the colorimetric sensor with a solution sample and utilizing an absorption measurement or a visual observation with the naked eye to detect color change in the polydiacetylene membrane.

Claims

1. A colorimetric sensor comprising polydiacetylene membrane liposomes, a polydiacetylene membrane film or fine particles coated with a polydiacetylene membrane, in which said polydiacetylene membrane is incorporated with a protein having a reduced molecular weight low enough not to cause color change in the polydiacetylene membrane, wherein said reduced-molecular-weight protein is a combination of single-stranded DNA of 100 bases or less which hybridizes with single-stranded DNA contained in a sample to form a double-stranded DNA, and an antibody which reacts with said double-stranded DNA but does not react with the single-stranded DNA contained in the sample.