Method and apparatus for detecting an organism-originated substance

• US 6,277,654 B1
• Filed: 03/30/2000
• Issued: 08/21/2001
• Est. Priority Date: 03/30/1999
• Status: Expired due to Fees

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Tsutamori, Yasuhiro
• Primary Examiner(s): Le, Long V.
• Assistant Examiner(s): Padmanabhan, Kartic
• Application Number:
  US09/539,410
• Time in Patent Office: 509 Days
• Field of Search: 435/6, 435/7.1, 435/4, 435/7.2, 435/7.4, 435/7.5, 435/7.6, 435/7.7, 435/7.71, 435/7.72, 435/7.8, 435/7.92, 435/91.1, 435/91.2, 435/69.1, 435/810, 435/285.1, 435/287.2, 436/501, 436/518, 430/5, 422/50, 422/68.1, 536/24.33, 536/23.1, 536/24.31, 536/24.32, 536/24.3, 536/24.1, 536/25.3
• US Class Current: 436/518
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

Various types of templates are stored in a template storage section in accordance with various types of array chips varying in disposition of spot positions. In making an analysis in an analysis section, a template, on which ROIs are disposed at positions corresponding to the spot positions on the array chip used, is read out from the template storage section. This template is set onto images represented by labeled data, and a signal value at a position corresponding to the ROI on the template is detected and evaluated.

Claims

1. A method for detecting an organism-originated substance comprising the steps of:

respectively binding organism-originated substances of a subject labeled with a labeling substance to a plurality of known specific binding substances differing from one another, the specific binding substances being disposed at a plurality of predetermined positions on a carrier on an array chip;

obtaining an image represented by labeling signals emitted from the labeling substances of said organism-originated substances bound to said specific binding substances;

setting a template of a type corresponding to said array chip onto said image, the template having regions of interest disposed at positions corresponding to said plurality of predetermined positions on said array chip where the specific binding substances are disposed; and

detecting values of said labeling signals at positions on said image which correspond to said regions of interest;

wherein said step of setting the template comprises reading out one template from a plurality of templates stored in a template storage means, and based on said template read out, said labeling values are detected.

2. The method as set forth in claim 1, further comprising the step of generating a new template and storing said new template in said template storage means.

3. The method according to claim 1, further comprising a step of adjusting at least one of a template orientation and a template size such that the regions of interest of said template overlap with labeling signals emitted from the labeling substances of said organism-originated substances bound to said specific binding substances.

4. The method according to claim 1, further comprising steps of:

assigning an identifier to each region of interest; and

selectively displaying, for one or more regions of interest, the identifier, the labeling signal information and binding substance characteristic information corresponding to the region of interest.