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Computer-assisted isolation and characterization of proteins

  • US 6,278,794 B1
  • Filed: 02/08/2000
  • Issued: 08/21/2001
  • Est. Priority Date: 11/29/1996
  • Status: Expired due to Fees
First Claim
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1. A computer-assisted method for isolating one or more selected proteins present in a two-dimensional array prepared by two-dimensional electrophoresis in a polyacrylamide gel, the method comprising:

  • (a) imaging the two-dimensional array or a replica thereof with a first device to generate a computer-readable output;

    (b) processing the output in at least one computer to select a protein for isolation;

    (c) generating machine-readable instructions that direct a second device to isolate the selected protein from the two-dimensional array; and

    (d) isolating the selected protein with the second device in accordance with the machine-readable instructions by removing a portion of the polyacrylamide gel containing the selected protein, wherein the second device is physically separate from the first device.

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