Compositions containing thrombin and microfibrillar collagen and methods for preparation and use thereof
First Claim
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1. A hemostatic composition comprising thrombin and microfibrillar collagen in an aqueous medium, wherein the microfibrillar collagen has an average diameter of about 3-30 nm.
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Abstract
The present invention relates to thrombin-containing hemostatic compositions, their preparation and use. In particular, it relates to hemostatic compositions comprising stabilized thrombin and microfibrillar collagen in an aqueous medium. In a preferred embodiment of the present invention, the compositions are used in a kit comprising two different components, one of which is autologous patient'"'"'s plasma as the source of fibrinogen, and the other of which is the thrombin-containing composition which also contains microfibrillar collagen.
128 Citations
42 Claims
- 1. A hemostatic composition comprising thrombin and microfibrillar collagen in an aqueous medium, wherein the microfibrillar collagen has an average diameter of about 3-30 nm.
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32. A method for promoting hemostasis of a tissue site on or in a body of a mammalian subject comprising the steps of:
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(a) providing a hemostatic composition comprising thrombin and microfibrillar collagen in an aqueous medium, wherein the microfibrillar collagen has an average diameter of about 3-30 mn;
(b) providing an aqueous medium comprising fibrinogen;
(c) mixing the hemostatic composition and the aqueous medium comprising fibrinogen immediately prior to contact with the tissue site; and
(d) contacting the mixture with the tissue site. - View Dependent Claims (33, 34, 35, 36, 37)
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38. A method for promoting hemostasis of a tissue site on or in a body of a mammalian subject comprising the steps of:
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(a) providing a hemostatic composition comprising thrombin and microfibrillar collagen in an aqueous medium, wherein the microfibrillar collagen has an average diameter of about 3-30 nm;
(b) providing an aqueous medium comprising platelets;
(c) mixing the hemostatic composition and the aqueous medium comprising platelets immediately prior to contact with the tissue site; and
(d) contacting the mixture with the tissue site.
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39. A method of making a composition for use as a hemostatic agent comprising the steps of:
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(a) mixing thrombin, fibrillar collagen and a fiber disassembly agent together in an aqueous medium, wherein the fiber disassembly agent is present at a concentration which is effective at disassembling the fibrillar collagen into microfibrillar collagen; and
(b) incubating the mixture formed in step (a) for a sufficient length of time and at a sufficient temperature to form microfibrillar collagen having a stable fiber size wherein the stable fiber size is about 3-30 nm in average diameter. - View Dependent Claims (40, 41, 42)
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Specification