Electrochemical affinity assay
First Claim
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1. An assay system for detecting a second member of a ligand—
- ligand receptor pair, the system comprising;
a) an electrode coated with a film comprising a redox polymer;
b) a first member of a ligand—
ligand receptor pair that binds the second member, wherein the first member is immobilized in the redox polymer; and
c) a third member that binds the second member, the third member being labeled with a detection marker;
wherein binding of the second member to the first member and of the third member to the second member results in increased rate of oxidation or reduction of a detection compound, when a potential is applied to the electrode.
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Abstract
An electrochemical affinity assay system for detection of ligand—ligand receptor binding.
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Citations
17 Claims
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1. An assay system for detecting a second member of a ligand—
- ligand receptor pair, the system comprising;
a) an electrode coated with a film comprising a redox polymer;
b) a first member of a ligand—
ligand receptor pair that binds the second member, wherein the first member is immobilized in the redox polymer; and
c) a third member that binds the second member, the third member being labeled with a detection marker;
wherein binding of the second member to the first member and of the third member to the second member results in increased rate of oxidation or reduction of a detection compound, when a potential is applied to the electrode. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
- ligand receptor pair, the system comprising;
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16. An assay system for detecting a second member of a ligand—
- ligand receptor pair, the system comprising;
a) an electrode coated with a film comprising a redox polymer;
b) a first member of a ligand—
ligand receptor pair that binds the second member, wherein the first member is immobilized in the redox polymer;
c) a third member that binds the second member, the third member being labeled with a detection marker; and
d) a substrate-generating unit located within the redox polymer to generate a substrate of the detection marker, wherein binding of the second member to the first member and of the third member to the second member results in increased rate of oxidation or reduction of the detection compound, when a potential is applied to the electrode.
- ligand receptor pair, the system comprising;
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17. An assay system comprising:
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a) an electrode coated with a film comprising a redox polymer;
b) a first member of a ligand—
ligand receptor pair immobilized in the redox polymer;
c) a second member of the ligand—
ligand receptor pair that binds the first member;
d) a third member that binds the second member, the third member being labeled with a detection marker; and
e) a detection compound generated by electrooxidation or electroreduction within the film, wherein the detection compound comprises a substrate of the detection marker, wherein binding of the second member to the first member and of the third member to the second member results in increased rate of oxidation or reduction of the detection compound, when a potential is applied to the electrode.
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Specification