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Electrochemical affinity assay

  • US 6,281,006 B1
  • Filed: 08/24/1998
  • Issued: 08/28/2001
  • Est. Priority Date: 08/24/1998
  • Status: Expired due to Term
First Claim
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1. An assay system for detecting a second member of a ligand—

  • ligand receptor pair, the system comprising;

    a) an electrode coated with a film comprising a redox polymer;

    b) a first member of a ligand—

    ligand receptor pair that binds the second member, wherein the first member is immobilized in the redox polymer; and

    c) a third member that binds the second member, the third member being labeled with a detection marker;

    wherein binding of the second member to the first member and of the third member to the second member results in increased rate of oxidation or reduction of a detection compound, when a potential is applied to the electrode.

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