Enclosed living cell dispensing tube

• US 6,283,294 B1
• Filed: 09/01/1999
• Issued: 09/04/2001
• Est. Priority Date: 09/01/1999
• Status: Expired due to Term

• Current Assignee: Biogaia Biologics AB
• Original Assignee: Biogaia Biologics AB
• Inventors: Skolling, Otto, Thorball, Jørgen, Casas, Ivan A.
• Primary Examiner(s): Gehman, Bryon P.
• Application Number:
  US09/387,947
• Time in Patent Office: 734 Days
• Field of Search: 206/219, 206/484, 206/438, 239/33, 426/85, 426/87, 426/115, 424/439, 424/473, 424/93.45, 604/518, 604/85
• US Class Current: 206/438
• CPC Class Codes: A23L 2/52 Adding ingredients adding preservatives A23L2/44
• CPC Class Codes: A47G 21/183 with means for changing the flavour of the liquid
• CPC Class Codes: A61J 1/201 having one piercing end
• CPC Class Codes: A61J 1/2089 Containers or vials which are to be joined to each other in order to mix their contents combinations of vial and syringe for mixing their contents A61J1/2096
• CPC Class Codes: A61M 5/1407 Infusion of two or more substances
• CPC Class Codes: B65D 81/32 for packaging two or more different materials which must be maintained separate prior to use in admixture containers with removable or destructible partitions B65D25/08 ; closures combined with auxiliary containers B65D51/28; in aerosol containers B65D83/682; amalgam capsules A61C5/66; containers for pharmaceutical purposes A61J1/20, A61M5/19; medical syringes A61M3/005, resin cartridges for grouting anchoring-bolts in mines E21D20/026

Abstract

A dispensing tube containing a selected material, such as bacterial cells or other additive, on the inside of the tube. The tube is wrapped and sealed in an outer watertight envelope until time for usage. At the time of usage the outer envelope is taken away and when the tubular device penetrates a solution container such as a beverage or an enteral solution, the selected material is added to the solution while the solution flows through the tube.

Claims

1. A device for dispensing living cells of a probiotic microorganism into a liquid. comprising:

(a) a liquid impermeable dispensing tube having an open bore being surrounded by an inner tube wall and extending from an open upper end of the tube to an open lower end of the tube;

(b) a coating material on a portion of the inner tube wall, said coating material selected from the group consisting of oils and waxes and holding a suspension of the living cells of the probiotic microorganism for long-term storage within the open bore adherent to the inner tube wall; and

(c) a flexible, essentially water vapor tight envelope enclosing the tube for long-term storage and to protect the living cells of the probiotic microorganism from moisture;

wherein the living cells of the probiotic microorganism may be removed from the inner tube wall and mixed with the liquid by placing the open lower end of the tube in the liquid and drawing the liquid through the bore.

2. The device of claim 1, wherein the probiotic microorganism comprises Lactobacillus.

3. The device of claim 1, wherein the cells of a probiotic microorganism comprise a suspension of lyophilized live bacteria.

4. The device of claim 3, in which the concentration of live bacteria at the point of use is at least 1% of the suspension when the device is manufactured.

5. The device of claim 1, wherein the viscosity of the suspension is modified to provide maximum adherence of the suspension to the device.

6. The device of claim 1, wherein the tube comprises a bellows portion.

7. The device of claim 1, wherein the tube telescopes into an outer tube.

8. The device of claim 1, wherein the tube is made from a synthetic polymer material.

9. The device of claim 1, wherein the tube is made from paper with an internal coating of a wax material.

10. The device of claim 1, wherein the tube has a diameter ranging from 0.2-20 mm and a length of 50-500 mm.

11. The device of claim 1, wherein the tube is a commercial drinking straw.

12. The device of claim 1, wherein the open bore of the tube has been surface modified to give a higher surface tension.

13. The device of claim 1, wherein the envelope is made of a polyolefinic material coated with aluminum.

14. The device of claim 1, wherein the envelope is made of a synthetic polymer with a low water permeation rate.

15. The device of claim 1, wherein the suspension of cells is distributed along the full length of the inner tube wall.

16. The device of claim 1, wherein the suspension of cells is primarily placed at the lower end of the tube.

17. The device of claim 1, wherein the coating material comprises a dietary oil.

18. The device of claim 1, wherein the envelope comprises an aluminum layer.

19. A method of making a device for dispensing living cells of a probiotic microorganism into a liquid, comprising:

(a) providing a liquid impermeable dispensing tube having an open bore being surrounded by an inner tube wall and extending from an open upper end of the tube to an open lower end of the tube;

(b) coating a portion of the inner tube wall within the open bore with a coating material selected from the group consisting of oils and waxes and holding a suspension of the living cells of the probiotic microorganism for long-term storage so that the coating material containing the living cells adheres to the inner tube wall; and

(c) providing a flexible, essentially water vapor tight envelope enclosing the tube for long-term storage and to protect the living cells of the probiotic microorganism from moisture;

wherein the living cells of the probiotic microorganism may be removed from the inner tube wall and mixed with the liquid by placing the open lower end of the tube in the liquid and drawing the liquid through the bore.

20. The method of claim 19, wherein the inner tube wall is coated with the cells of the probiotic microorganism by insertion of a second tube that has a coating of the cells of the probiotic microorganism into the lower end of the dispensing tube.

21. The method of claim 19, wherein the probiotic microorganism comprises Lactobacillus.

22. The method of claim 19, wherein the cells of the probiotic microorganism comprise a suspension of lyophilized live bacteria.

23. The method of claim 22, in which the concentration of live bacteria at the point of use is at least 1% of the suspension when the device is manufactured.

24. The method of claim 19, wherein providing the liquid impermeable dispensing tube comprises providing a tube comprising a bellows portion.

25. The method of claim 19, wherein providing the liquid impermeable dispensing tube comprises providing a tube that telescopes into an outer tube.

26. The method of claim 19, wherein providing the liquid impermeable dispensing tube comprises providing a tube made from a synthetic polymer material.

27. The method of claim 19, wherein providing the liquid impermeable dispensing tube comprises providing a tube made from paper with an internal coating of a wax material.

28. The method of claim 19, wherein providing a liquid impermeable dispensing tube comprises providing a tube having a diameter ranging from 0.2-20 mm and a length of 50-500 mm.

29. The method of claim 19, wherein providing a liquid impermeable dispensing tube comprises providing a commercial drinking straw.

30. The method of claim 19, further comprising surface-modification of the open bore of the tube to give a higher surface tension.

31. The method of claim 19, wherein providing the flexible, essentially water vapor tight envelope enclosing the tube comprises providing a polyolefinic material coated with aluminum.

32. The method of claim 19, wherein providing the flexible, essentially water vapor tight envelope enclosing the tube comprises providing an envelope made of a synthetic polymer with a low water permeation rate.

33. The method of claim 19, wherein coating a portion of the inner tube wall comprises distributing the cells along the full length of the inner tube wall.

34. The method of claim 19, wherein coating a portion of the inner tube wall comprises distributing the suspension of cells at the lower end of the tube.

35. The method of claim 19, further comprising modifying the viscosity of the suspension to provide maximum adherence of the suspension to the device.

36. The method of claim 19, wherein the coating material comprises a dietary oil.

37. The method of claim 19, wherein the envelope comprises an aluminum layer.