Apparatus and method for transferring liquids

• US 6,284,113 B1
• Filed: 09/15/1998
• Issued: 09/04/2001
• Est. Priority Date: 09/19/1997
• Status: Expired due to Term

• Current Assignee: Monogram Biosciences, Inc. (Laboratory Corporation Of America Holdings)
• Original Assignee: Aclara Biosciences, Inc (Laboratory Corporation Of America Holdings)
• Inventors: Smith, Timothy F., Bjornson, Torleif Ove
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): STARSIAK, JOHN S
• Application Number:
  US09/153,814
• Time in Patent Office: 1,085 Days
• Field of Search: 422/100, 435/287.1, 435/287.2, 738/633.2, 738/633.1, 738/64, 738/640.1, 204/450, 204/451, 204/453, 204/600, 204/601, 204/604
• US Class Current: 204/453
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00358 electrode driven
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 2035/1037 Using surface tension, e.g. pins or wires
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: G01N 35/1074 arranged in a two-dimensional array

Abstract

The present invention concerns devices, apparatus and methods for transferring liquids. One aspect of the present invention is a device comprising a plate having a plurality of transfer elements. Each of the transfer elements comprises an aperture in the plate where the aperture is capable of being electrically activated. The plate has one of more attaching elements for attaching the plate to a multiwell plate to form a sealed system except for the apertures of the transfer elements. Usually, the device is adapted for sealing attachment to a multiwell plate. In a method in accordance with the present invention a quantity of liquid is disposed to a second side of a plate having a plurality of apertures in the plate. The apertures are capable of being electrically activated. The liquid is present in a closed well except for the apertures in the plate. To simultaneously expel liquid from the apertures, the apertures are electrically activated. Also disclosed are kits comprising a device in accordance with the present invention.

Claims

1. A device comprising:

(a) a first plate having a plurality of transfer elements, each transfer element comprising an aperture in said plate wherein said aperture is capable of being electrically activated;

(b) a multiwell plate comprising a plurality of wells;

(c) one or more attaching elements on a first side of said first plate for sealingly attaching said first plate to said multiwell plate to form a sealed system except for said apertures;

(d) electrodes for electrically activating said aperture.

2. The device of claim 1 wherein each of said transfer elements further comprises a nozzle on a second side of said plate as an extension of said aperture wherein said nozzle is formed from an electroconductive material.

3. The device of claim 2 wherein said electroconductive material is adapted for activation by an applied electric potential.

4. The device of claim 1 wherein said attaching element is removably attachable to said multiwell plate.

32. A kit comprising in packaged combination:

(a) the device of claim 1 and

(b) a sample receiving plate.

33. The kit of claim 32 wherein said sample receiving plate is selected from the group consisting of planar surfaces and sample receiving reservoirs.

34. The kit of claim 32 which comprises one or more reagents for conducting a chemical synthesis or an analysis.

35. The kit of claim 32 which comprises a multiwell plate.

5. An apparatus for transferring liquid, said apparatus comprising:

(a) a first plate comprising a plurality of individual wells containing said liquid, said wells being formed in said first plate on a first side thereof, wherein a second side of said first plate is free of openings;

(b) a second plate comprising a first side and a second side and a plurality of transfer elements, each transfer element comprising an aperture in said second plate, wherein said apertures are capable of being electrically activated, said second plate being adapted for simultaneously transferring precise amounts of a liquid from said first plate to a sample receiving plate by electrically activating said apertures, said second side of said second plate being sealingly attached to the first side of said first plate; and

(c) electrodes for electrically activating said aperture.

6. The apparatus of claim 5, further comprising a sample receiving plate, wherein said sample receiving plate comprises a plurality of sample receiving reservoirs that are part of a microfluidic network.

7. The apparatus of claim 6 wherein said apertures are comprised of an electroconductive material serving as a first electrode and said sample receiving plate has a second electrode adjacent thereto and cooperative with said first electrode to provide for application of an electric potential between said electroconductive material and said second electrode.

8. The apparatus of claim 5 wherein each of said transfer elements further comprises a nozzle on a second side of said plate as an extension of said aperture wherein said nozzle is formed from an electroconductive material.

9. The apparatus of claim 5 wherein said second side of said second plate comprises one or more friction members to render said second plate sealingly attached to the first side of said first plate.

10. The apparatus of claim 9 wherein said second plate is removably attached to the first side of said first plate.

11. The apparatus of claim 5 wherein said second side of said second plate comprises an adhesive layer to render said second plate sealingly attached to the first side of said first plate.

12. The apparatus of claim 5 which further comprises an acceleration electrode.

13. The apparatus of claim 5 which further comprises an array of capillaries.

14. A method for trnsfering liquid, said method comprising:

(a) disposing a quantity of liquid to a second side of a plate having a plurality of apertures therein that are capable of being electrically activated, wherein said liquid is present in a closed container comprising individual wells of a multiwell plate and

(b) electrically activating said apertures.

15. The method of claim 14 wherein said container is a well that is part of a 96 multiwell plate.

16. The method of claim 15 wherein said well of said multiwell plate comprises a bottom wall wherein said bottom wall is substantially free of contact with said liquid.

17. The method of claim 15 wherein said second side of said plate comprises one or more friction members to render said plate sealingly attached to said multiwell plate to form said closed system.

18. The method of claim 17 wherein said multiwell plate is removably attached to the first side of said first plate.

19. The method of claim 14 wherein said apertures are part of transfer elements, each of said transfer elements comprising a nozzle on a first side of said plate as an extension of said aperture wherein said nozzle is formed from an electroconductive material.

20. The method of claim 14 wherein said second side of said plate comprises an adhesive layer to render said plate sealingly attached to said multiwell plate to form said closed system.

21. A method for transferring liquid, said method comprising:

(a) providing a first plate comprising a plurality of individual wells containing said liquid, said wells being formed in said first plate on a first side thereof,

(b) providing a second plate comprising a first side and a second side and a plurality of transfer elements, each transfer element comprising an aperture in said second plate, wherein said apertures are capable of being electrically activated, said second plate being adapted for simultaneously transferring precise amounts of a liquid from said first plate to a sample receiving plate by electrically activating said apertures,

(c) sealingly attaching said second side of said second plate to the first side of said first plate,

(d) positioning said apertures adjacent to an array of sample receiving plate, and

(e) electrically activating said apertures.

22. The method of claim 21 wherein said sample receiving plate comprises a plurality of sample receiving reservoirs that are part of a microfluidic network.

23. The method of claim 21 wherein said apertures are comprised of an electroconductive material serving as a first electrode and said sample receiving plate has a second electrode adjacent thereto and cooperative with said first electrode to provide for application of an electric potential to electrically activate said apertures.

24. The method of claim 21 wherein each of said transfer elements further comprises a nozzle on a first side of said plate as an extension of said aperture wherein said nozzle is formed from an electroconductive material.

25. The method of claim 21 wherein said second side of said second plate comprises one or more friction members to render said second plate sealingly attached to the first side of said first plate.

26. The method of claim 25 wherein said second plate is removably attached to the first side of said first plate.

27. The method of claim 21 wherein said second side of said second plate comprises an adhesive layer to render said second plate sealingly attached to the first side of said first plate.

28. A method for transferring liquid, said method comprising:

(a) attaching, to a multiwell plate having liquid contained in the wells thereof, a second plate comprising a first side and a second side and a plurality of apertures therethrough, each of said apertures being at least partially comprised of an electroconductive material, each of said apertures being aligned with a corresponding well of said multiwell plate, said second plate comprising on said second side one or more attaching elements and said second plate being attached to the top of said multiwell plate by said attaching element,

(b) inverting said multiwell plate attached to said second plate so that liquid is disposed at each of said apertures, the dimensions of said apertures being such that liquid does not exit said apertures,

(c) positioning said apertures adjacent to an array of sample receiving reservoirs of a microfluidic network in a third plate wherein each of said microfluidic networks has an electrode connected to an electrode adjacent one of said apertures,

(d) applying an electric potential across said electrodes causing a portion of said liquid to exit said apertures and enter a corresponding sample receiving reservoir.

29. The method of claim 28 wherein said apertures are part of a transfer element, each of said transfer elements further comprising a nozzle on a first side of said plate as an extension of said aperture wherein said nozzle is formed from an electroconductive material.

30. The method of claim 28 wherein said attaching element comprises one or more friction members.

31. The method of claim 28 wherein said attaching element comprises an adhesive layer.