Electrode for the electrochemical detection of nitric oxide

• US 6,287,452 B1
• Filed: 09/20/1999
• Issued: 09/11/2001
• Est. Priority Date: 10/02/1996
• Status: Expired due to Fees

• Current Assignee: Duke University
• Original Assignee: Duke University
• Inventors: Coury, Louis A. Jr., Allen, Barry W., Piantadosi, Claude A.
• Primary Examiner(s): Bell, Bruce F.
• Application Number:
  US09/399,371
• Time in Patent Office: 722 Days
• Field of Search: 204/403, 204/415, 204/291, 205/777.5, 205/778, 205/781, 205/792, 205/793, 600/309, 600/345, 600/353
• US Class Current: 205/777.5
• CPC Class Codes: G01N 27/30 Electrodes, e.g. test electrodes; Half-cells G01N27/414 takes precedence

Abstract

Nitric oxide-specific electrodes are useful for in situ detection of nitric oxide in biomedical applications and have at least a surface region thereof which is capable of forming complexes with nitric oxide, for example, nitrosyl complexes. The nitric oxide complexes formed at the surface of the electrodes apparently increase the concentration of nitric oxide available for detection, thereby leading to significantly improved relative responses as compared to other known nitric oxide electrode materials. Most preferably, the electrode has at least an exterior surface region which contains ruthenium and/or at least one oxide of ruthenium. The electrodes are advantageously conditioned in saline solution at +675 mV for about two hours.

Claims

1. An electrode having a surface region comprised of a metal oxide capable of enhanced detection of nitric oxide when exposed to a nitric oxide-containing fluid.

2. The electrode of claim 1, wherein at least the surface region comprised of at least one oxide of ruthenium.

3. The electrode of claim 1, wherein said at least one surface region is comprised of at least one oxide of ruthenium having the formula RuO_x, where x is a number greater than 0.

4. The electrode of claim 3, wherein x is a number greater than 0 up to and including about 2.

5. The electrode of claim 1, wherein at least the surface region consists essentially of ruthenium conditioned at a potential for a time sufficient to cause the surface region to exhibit maximal nitric oxide response when exposed to the nitric oxide-containing fluid.

9. A medical device which comprises an electrode according to any one of claims 1-8.

10. The device of claim 9, adapted to detect nitric oxide in a biological fluid.

11. The device of claim 10, wherein the biological fluid is at least one of blood, urine and synovial fluid.

12. A medical device as in claim 9 adapted for the in vivo detection of nitric oxide in a biological fluid.

13. A method for the in vivo detection of nitric oxide comprising placing an electrode according to any one of claims 1-8 at a site within a patient or other living organism, and then determining the nitric oxide present at said site by the electrochemical response of said electrode.

14. A method of detecting nitric oxide in a biological sample comprising bringing an electrode according to any one of claims 1-8 into contact with the biological sample, and then determining the nitric oxide present in said sample by the electrochemical response of said electrode.

6. A nitric-oxide specific electrode having a surface region conditioned at a potential which enhances nitric oxide detection when the electrode is exposed to a nitric oxide-containing fluid.

7. The electrode of claim 6, wherein said at least one oxide of ruthenium is RuO_x, where x is a number greater than 0.

8. The electrode of claim 7, wherein where x is greater than 0 up to and including about 2.

15. A method of making an electrode for the detection of nitric oxide which comprises conditioning a metal electrode surface at a potential for a time sufficient for the surface to exhibit enhanced nitric oxide response.

16. The method as in claim 15, wherein the metal electrode surface is held at a potential for a time sufficient to allow electrode current to decay to a stable baseline.

17. An electrode for the detection of nitric oxide made according to claim 15 or 16.