Methods for detecting and identifying single molecules
First Claim
1. A method of detecting a single nonnaturally occurring oligonucleotide molecule capable of specifically binding to a selected nonoligonucleotide molecule comprising:
- (a) contacting a mixture comprising an assortment of nonnaturally occurring oligonucleotides with a selected nonoligonucleotide molecule; and
(b) detecting a single nonnaturally occurring oligonucleotide molecule specifically bound to said selected nonoligonucleotide molecule by at least one of scanning probe microscopy, optical trapping or flow cytometry.
3 Assignments
0 Petitions
Accused Products
Abstract
Multimolecular devices and drug delivery systems prepared from synthetic heteropolymers, heteropolymeric discrete structures, multivalent heteropolymeric hybrid structures, aptameric multimolecular devices, multivalent imprints, tethered specific recognition devices, paired specific recognition devices, nonaptameric multimolecular devices and immobilized multimolecular structures are provided, including molecular adsorbents and multimolecular adherents, adhesives, transducers, switches, sensors and delivery systems. Methods for selecting single synthetic nucleotides, shape-specific probes and specifically attractive surfaces for use in these multimolecular devices are also provided. In addition, paired nucleotide-nonnucleotide mapping libraries for transposition of selected populations of selected nonoligonucleotide molecules into selected populations of replicatable nucleotide sequences are described.
508 Citations
27 Claims
-
1. A method of detecting a single nonnaturally occurring oligonucleotide molecule capable of specifically binding to a selected nonoligonucleotide molecule comprising:
-
(a) contacting a mixture comprising an assortment of nonnaturally occurring oligonucleotides with a selected nonoligonucleotide molecule; and
(b) detecting a single nonnaturally occurring oligonucleotide molecule specifically bound to said selected nonoligonucleotide molecule by at least one of scanning probe microscopy, optical trapping or flow cytometry. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
-
12. A method of detecting a single nonnaturally occurring oligonucleotide molecule capable of specifically binding to a selected nonoligonucleotide molecule comprising:
-
(a) preparing a reaction mixture comprising a selected nonoligonucleotide molecule and a nonnaturally occurring oligonucleotide comprising an unknown nucleotide sequence; and
(b) detecting a single nonnaturally occurring oligonucleotide molecule specifically bound to said selected nonoligonucleotide molecule by at least one of scanning probe microscopy, optical trapping or flow cytometry. - View Dependent Claims (13, 14, 15)
-
-
16. A method of detecting a single nonnaturally occurring oligonucleotide molecule capable of specifically binding to a selected target that is not a DNA-binding protein comprising:
-
(a) selecting a target that is not a DNA-binding protein;
(b) contacting the selected target with a mixture comprising an assortment of nonnaturally occurring oligonucleotides; and
(c) detecting a single nonnaturally occurring oligonucleotide molecule specifically bound to said selected target by at least one of scanning probe microscopy, optical trapping or flow cytometry. - View Dependent Claims (17, 18, 19)
-
-
20. A method of detecting a single nonnaturally occurring oligonucleotide conjugate molecule capable of specifically binding to a selected nonoligonucleotide molecule, said method comprising:
-
(a) preparing a reaction mixture comprising a selected nonoligonucleotide molecule and a plurality of different nonnaturally occurring oligonucleotide conjugates, each different nonnaturally occurring oligonucleotide conjugate comprising a different nonnucleotide moiety attached to an oligonucleotide; and
(b) detecting a single nonnaturally occurring oligonucleotide conjugate molecule specifically bound to said selected nonoligonucleotide molecule by at least one of scanning probe microscopy, optical trapping or flow cytometry. - View Dependent Claims (21, 22, 23, 24)
-
-
25. A method of detecting a single nonoligonucleotide molecule comprising:
-
(a) selecting a nonnaturally occurring oligonucleotide capable of specifically binding to a selected nonoligonucleotide molecule;
(b) preparing a reaction mixture comprising said nonnaturally occurring oligonucleotide and said selected nonoligonucleotide molecule; and
(c) detecting a single complex comprising said nonnaturally occurring oligonucleotide specifically bound to said selected nonoligonucleotide molecule by at least one of scanning probe microscopy, optical trapping or flow cytometry. - View Dependent Claims (26, 27)
-
Specification