DNA probe array

• US 6,288,220 B1
• Filed: 03/02/1999
• Issued: 09/11/2001
• Est. Priority Date: 03/05/1998
• Status: Expired due to Term

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Kambara, Hideki, Okano, Kazunori
• Primary Examiner(s): Jones, W. Gary
• Assistant Examiner(s): Taylor, Janell E.
• Application Number:
  US09/260,543
• Time in Patent Office: 924 Days
• Field of Search: 536/24.31, 536/24.3, 536/24.32, 536/24.33, 536/25.3, 436/518, 436/523, 436/172, 436/805, 436/809, 422/50, 422/55, 422/64, 422/67, 422/82.05, 422/82.11, 422/99, 435/6, 435/7.1, 435/7.5, 204/406
• US Class Current: 536/24.31
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00459 Beads
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/0052 in the shape of elongated tubes
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00628 Ionic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00657 One-dimensional arrays
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: Y10S 436/805 : Optical property
• CPC Class Codes: Y10S 436/809 : Multifield plates or multicontainer arrays

Abstract

There is used at least one probe array obtained by arraying particles having various probes, respectively, fixed thereon (probe particles) in a definite order in a holder. A plurality of capillaries or grooves packed with various kinds, respectively, of probe particles are arrayed in parallel, and one of particles contained in each capillary or groove is injected into another capillary or groove to produce a probe array in which the various kinds of probe particles are arrayed in a constant and definite order. Various fluorophore-labeled DNA'"'"'s are measured at the same time by attaching various probes to particles, respectively, of different sizes. A probe array composed of various fixed DNA probes can easily be produced, and there can be provided a probe array for detecting various DNA'"'"'s which is composed of various fixed arbitrary DNA probes.

Claims

1. A probe array for analyzing target substances which comprises an array of a plurality of spherical particles having probes, respectively, fixed thereon, said probes being capable of hybridizing to different target substances to be analyzed, respectively, and said particles are arrayed in a single straight line in a transparent capillary, wherein the species of each of said probes can be known from the place of each of said particles in the order of each of said particles arrayed in said single straight line.

2. A probe array for analyzing target substances which comprises a plurality of spherical particles having probes, respectively, fixed thereon, said probes being capable of hybridizing to different target substances to be analyzed, respectively, wherein said particles are arrayed in a single straight line in a predetermined order in a transparent capillary, and said order is such that the arraying positions in said straight line of said particles correspond to the species, respectively, of said probes fixed on said particles.

3. A probe array according to claim 2, wherein said particles holding said probes are labeled with different dyes or fluorophores, respectively, depending on the species of said probes held by the particles.

4. A probe array according to claim 2, wherein said particles holding said probes are two-dimensionally arrayed at predetermined positions by arraying a plurality of capillaries holding said particles holding said probes.

5. A probe array for analyzing target substances which comprises a plurality of spherical particles having probes, respectively, fixed thereon, said probes being capable of hybridizing to different target substances to be analyzed, respectively, and said particles are arrayed in a single straight line in a transparent capillary, wherein said particles are arrayed so that positions of said particles in the single straight line may correspond to the species, respectively, of said probes.

6. A probe array according to claim 5, wherein said particles holding said probes are labeled with different dyes or fluorphores, respectively, depending on the species of said probes held by the particles.

7. A probe array according to claim 5, wherein said particles holding said probes are two-dimensionally arrayed at predetermined positions by arraying a plurality of capillaries holding said particles holding said probes.

8. A probe array which comprises an array of a plurality of small spherical particles having probes, respectively, thereon, wherein said particles are arrayed in a single straight line in each of a plurality of transparent capillaries and said probes are arrayed two-dimensionally, and wherein an order of arrangement in said single straight line of the small spherical particles having probes is predetermined, or positions of arrangement in said single straight line of the small spherical particles having probes are predetermined.

9. A probe array according to claim 8, wherein marker particles are placed between the small spherical particles having different species of probes.

10. A probe array according to claim 8, wherein species of the probes on each of the small spherical particles are different from each other.

11. A probe array according to claim 8, wherein the small spherical particles include particles of the same species as the probes.

12. A probe array according to claim 8, wherein each of the probes is capable of binding to DNA, RNA or a protein.

13. A probe array according to claim 8, wherein the small spherical particles are spherical plastic particles.

14. A probe array for analyzing target substances which comprises a plurality of spherical particles having probes, respectively, fixed thereon, said probes being capable of hybridizing to different target substances to be analyzed, respectively, and said particles being arrayed in a single straight line in a transparent capillary, wherein an inside diameter of said capillary is too small for the passage of two particles and is sufficient for the passage of one particle.