Sample injector for high pressure liquid chromatography

• US 6,290,909 B1
• Filed: 04/13/2000
• Issued: 09/18/2001
• Est. Priority Date: 04/13/2000
• Status: Expired due to Term

• Current Assignee: National Technology & Engineering Solutions Of Sandia LLC (Honeywell International Inc.)
• Original Assignee: Sandia Corporation (Martin Marietta Corporation)
• Inventors: Paul, Phillip H., Arnold, Don W., Neyer, David W.
• Primary Examiner(s): LUDLOW, JAN M
• Application Number:
  US09/548,475
• Time in Patent Office: 523 Days
• Field of Search: 422/70, 422/89, 422/100, 95/89, 961/05, 436/161, 73/234.1, 73/615.5, 73/615.6, 210/198.2, 210/656, 210/659, 204/450, 204/600, 204/647, 204/648
• US Class Current: 422/70
• CPC Class Codes: G01N 2030/285 electrically driven carrier
• CPC Class Codes: G01N 30/22 in high pressure liquid systems
• CPC Class Codes: G01N 30/6095 Micromachined or nanomachined, e.g. micro- or nanosize
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

Apparatus and method for driving a sample, having a well-defined volume, under pressure into a chromatography column. A conventional high pressure sampling valve is replaced by a sample injector composed of a pair of injector components connected in series to a common junction. The injector components are containers of porous dielectric material constructed so as to provide for electroosmotic flow of a sample into the junction. At an appropriate time, a pressure pulse from a high pressure source, that can be an electrokinetic pump, connected to the common junction, drives a portion of the sample, whose size is determined by the dead volume of the common junction, into the chromatographic column for subsequent separation and analysis. The apparatus can be fabricated on a substrate for microanalytical applications.

Claims

1. An apparatus for injecting a sample into a chromatographic column, comprising:

a source of high pressure joined at a common junction to the inlet of the chromatographic column; and

a sample injector joined to the common junction, said sample injector comprising at least two elements combined in a series arrangement and connected to a common power supply, wherein each element comprises a container having an inlet end and an outlet end, the container filled with a dielectric material forming a porous bed therein.

2. The apparatus of claim 1, wherein said high pressure source comprises an electrokinetic pump.

3. The apparatus of claim 1, wherein the porous dielectric material is comprised of a nonporous and uncoated material.

4. The apparatus of claim 3, wherein the dielectric material includes a glass, ceramic, or polymer material.

5. The apparatus of claim 4, wherein the dielectric material is silica beads.

6. The apparatus of claim 1, wherein the porous bed has a pore size in the range of about 25 to 300 nm.

7. The apparatus of claim 1, wherein the power supply includes electrodes and further including at least one salt bridge disposed between the electrodes of the power supply and the elements of said sample injector.

8. An apparatus for the high pressure injection of a sample into a chromatography column, comprising:

a substrate fabricated to define a microchannel system disposed thereon, the microchannel system comprising, in combination,

a source of high pressure joined at a common junction to the inlet of the chromatography column; and

a sample injector joined to the to the common junction, said sample injector comprising at least two elements combined in a series arrangement and connected to a common power supply, wherein each element comprises a container having an inlet end and an outlet end, the container filled with a dielectric material forming a porous bed therein.

9. The apparatus of claim 8, wherein the source of high pressure is an electrokinetic pump.

10. The apparatus of claim 8, wherein the power supply includes electrodes and further including at least one salt bridge disposed between the electrodes of the power supply and the elements of said sample injector.

11. The apparatus of claim 8, wherein the dielectric material comprising the porous bed is produced by lithographic etching.