Nucleic acid purification and process
First Claim
1. A method for binding a nucleotide polymer to silicon carbide comprising the step of contacting said nucleotide polymer with silicon carbide.
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Abstract
The invention disclosed is particularly directed to a process for the purification of DNA, and is especially useful for the isolation of biologically active plasmid DNA. The process is initiated by binding the DNA to silicon carbide particles, either in the presence or absence of chaotropic agents. The bound DNA is subsequently eluted from the resin in low salt buffer or water. The resulting purified DNA is substantially free of proteins and is shown to be biologically active. The scope and diversity of the process is demonstrated here since this process can be used for the isolation of RNA and genomic DNA as well as the purification of PCR products and isolation of DNA from agarose gel slices.
145 Citations
18 Claims
- 1. A method for binding a nucleotide polymer to silicon carbide comprising the step of contacting said nucleotide polymer with silicon carbide.
Specification
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- Resources
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Current AssigneeNorgen Biotek Corp.
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Original AssigneeNorgen Biotek Corp.
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InventorsHaj-Ahmad, Yousef
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Primary Examiner(s)Sisson, Bradley L.
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Application NumberUS09/658,808Time in Patent Office372 DaysField of Search435/6, 435/94, 435/287.2, 536/23.1, 536/24.3, 536/24.33, 436/94US Class Current436/94CPC Class CodesC12N 15/1006 by means of a solid support...Y10T 436/143333 Saccharide [e.g., DNA, etc.]
