Processing for attaching an end of a nucleic acid to a surface by utilizing pH
First Claim
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1. A process for attaching an end of a nucleic acid on a surface comprising:
- (a) providing a solution having a pH that allows for direct attachment of an end of a nucleic acid to a surface;
(b) contacting the surface, the solution, and the nucleic acid to directly attach the end of the nucleic acid to the surface, wherein the remainder of the nucleic acid exists freely in solution.
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Abstract
The invention relates to a process of attaching an end of a nucleic acid on a surface by providing a solution having a pH that allows for specific attachment of an end of a nucleic acid to a surface. The surface, the solution, and the nucleic acid are contacted to attach the end of the nucleic acid to the surface.
61 Citations
41 Claims
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1. A process for attaching an end of a nucleic acid on a surface comprising:
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(a) providing a solution having a pH that allows for direct attachment of an end of a nucleic acid to a surface;
(b) contacting the surface, the solution, and the nucleic acid to directly attach the end of the nucleic acid to the surface, wherein the remainder of the nucleic acid exists freely in solution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 38, 39, 40, 41)
(a) providing the product of claim 1;
(b) contacting a sample containing a biological molecule with the product of claim 1; and
(c) detecting the presence or absence of binding of the biological molecule to the attached nucleic acid.
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11. The process according to claim 10, wherein the biological molecule is DNA, RNA, or protein.
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38. The process according to one of claims 1, 12, 21, or 29, wherein the surface is an organic or inorganic polymer.
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39. The process according to one of claims 1, 12, 21, or 29, wherein the surface is a metal or a metal oxide.
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40. The process according to one of claims 1, 12, 21, or 29, wherein the surface comprises a plate, bead, fiber or particle.
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41. The process according to one of claims 1, 12, 21, or 29, wherein the attached nucleic acid is complementary to a nucleic acid sequence to be isolated from a sample.
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12. A process for attaching an end of a nucleic acid on a surface comprising:
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(a) providing a solution having a pH of about 5 to about 10 that allows for direct attachment of an end of a nucleic acid to a surface;
(b) contacting the surface, the solution, and the nucleic acid to directly attach the end of the nucleic acid to the surface, wherein the remainder of the nucleic acid exists freely in solution. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20)
(a) providing the product of claim 12;
(b) contacting a sample containing a biological molecule with the product of claim 12; and
(c) detecting the presence or absence of binding of the biological molecule to the attached nucleic acid.
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20. The process according to claim 19, wherein the biological molecule is DNA, RNA, or protein.
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21. A process for attaching an end of a nucleic acid on a surfacing comprising:
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(a) providing a solution having a pH of about 5 to about 6 that allows for direct attachment of an end of a nucleic acid to a surface;
(b) contacting the surface, the solution, and the nucleic acid to directly attach the end of the nucleic acid to the surface, wherein the remainder of the nucleic acid exists freely in solution. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28)
(a) providing the product of claim 21;
(b) contacting a sample containing a biological molecule with the product of claim 21; and
(c) detecting the presence or absence of binding of the biological molecule to the attached nucleic acid.
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28. The process according to claim 27, wherein the biological molecule is DNA, RNA, or protein.
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29. A process for attaching an end of a nucleic acid on a surface comprising:
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(a) providing a solution having a pH of about 5 to about 8 that allows for attachment of an end of a nucleic acid to a surface;
(b) contacting the surface, the solution, and the nucleic acid to attach the end of the nucleic acid to the surface, wherein the remainder of the nucleic acid exists freely in solution. - View Dependent Claims (30, 31, 32, 33, 34, 35, 36, 37)
(a) providing the product of claim 29;
(b) contacting a sample containing a biological molecule with the product of claim 29; and
(c) detecting the presence or absence of binding of the biological molecule to the attached nucleic acid.
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37. The process according to claim 36, wherein the biological molecule is DNA, RNA, or protein.
Specification