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Methods for assessing genetic and phenotypic markers by simultaneous multicolor visualization of chromogenic dyes using brightfield microscopy and spectral imaging

  • US 6,294,331 B1
  • Filed: 08/07/1998
  • Issued: 09/25/2001
  • Est. Priority Date: 08/08/1997
  • Status: Expired due to Term
First Claim
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1. A method for detecting a genetic marker in a biological sample comprising:

  • a) contacting the biological sample with a nucleic acid probe linked to a detectable moiety, whereby the detectable moiety can be detected by the presence of a chromogenic dye associated with the detectable moiety;

    b) obtaining a spectral image of the biological sample using brightfield microscopy; and

    c) detecting the presence of the chromogenic dye, thereby detecting the genetic marker in the biological sample.

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