Spatially-encoded analyte detection

• US 6,294,392 B1
• Filed: 07/21/1999
• Issued: 09/25/2001
• Est. Priority Date: 07/21/1999
• Status: Expired due to Fees

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Brazill, Sara Ann, Kuhr, Werner G., Singhal, Pankaj
• Primary Examiner(s): Brusca, John S.
• Assistant Examiner(s): Lundgren, Jeffrey S.
• Application Number:
  US09/358,204
• Time in Patent Office: 797 Days
• Field of Search: 435/6, 205/775, 436/44, 436/518
• US Class Current: 436/518
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: G01N 27/447 using electrophoresis
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

A flow-through microchannel (e.g. capillary) biosensor is described for the for the detection of multiple, different analytes (e.g. nucleic acids, proteins, sugars, etc.) targets in a sample by binding them to “complementary” binding partners (e.g. complementary nucleic acids, ligands, antibodies, etc.). The binding partners are immobilized in different sections of a microchannel (e.g. a fused silica capillary). After fabrication of the biosensor, a sample is flushed through the capillary, and any target analyte(s) contained within the sample are bound to the immobilized binding partner(s) on the microchannel wall forming bound complexes. Finally, the bound complexes are simultaneously denatured along the entire length of the capillary and flushed out past a detector poised downstream, and the analyte concentration is measured (e.g., using sinusoidal voltammetry). Direct electrochemical detection of underivatized DNA is accomplished by oxidizing its sugar backbone and the amine containing nucleobase at the copper electrode. The elution time of the desorbed target DNA(s) is used for the sequence identification of the target. Multiple genetic sequences can be diagnosed by using a single biosensor in this manner. The sensor is highly specific due to hybridization chemistry, and extremely sensitive due to electrochemical detection.

Claims

1. A device for detecting a two or more analytes in a sample, said device comprising:

a channel having affixed therein a binding partner for each of said two or more analytes, where the binding partners for each of said two or more analytes are located in different regions of said channel prior to sample introduction and said channel has a cross-sectional area small enough such that when analytes are released from said two or more binding partners into a fluid flowing through said channel, said analytes remain spatially segregated until they reach a detection point along said channel downstream from said binding partners; and

a detector that detects said analytes within said channel at said detection point.

2. The device of claim 1, wherein said channel is a capillary tube.

3. The device of claim 2, wherein said capillary tube is a capillary electrophoresis tube.

4. The device of claim 1, wherein said channel is a channel etched in a surface.

5. The device of claim 4, wherein said channel is a channel etched in a glass surface.

6. The device of claim 1, wherein said channel has a cross-sectional area that provides a Renold'"'"'s number (Re) of less than about 1.

7. The device of claim 1, wherein said channel has a cross-sectional diameter less than about 100 μ

m.

8. The device of claim 1, wherein said two or more target analytes comprise at least three different analytes.

9. The device of claim 1, wherein said binding partners are selected from the group consisting of antibodies, binding proteins, and nucleic acids.

10. The device of claim 9, wherein said binding partners are nucleic acids.

11. The device of claim 1, wherein said detector comprises an absorbance spectrometer.

12. The device of claim 1, wherein said detector comprises a sinusoidal voltammeter.

13. A kit for the detection of two or more target analytes in a fluid, said kit comprising a channel having affixed therein a binding partner for each of said two or more analytes, where the binding partners for each of said two or more analytes are located in different regions of said channel prior to sample introduction and said channel has a cross-sectional area small enough such that when analytes are released from said two or more binding partners into a fluid flowing through said channel, said analytes remain spatially segregated until they reach a detection point in said channel downstream from said binding partners.

14. The kit of claim 13, wherein said kit comprises a plurality of said channel.

15. The kit of claim 14, wherein the channels comprising the plurality of channel each have a unique collection of binding partners.

16. The kit of claim 14, wherein said channel is a capillary tube.

17. The kit of claim 16, said capillary tube is a capillary electrophoresis tube.

18. The kit of claim 14, wherein said channel is a channel etched in a surface.

19. The kit of claim 18, wherein said channel is a channel etched in a glass surface.

20. The kit of claim 14, wherein said channel has a cross-sectional area that provides a Renold'"'"'s number (Re) of less than about 1.

21. The kit of claim 14, wherein said channel has a cross-sectional diameter less than about 100 μ

m.

22. The kit of claim 14, wherein said channel contains at least 3 different species of binding partner.

23. The kit of claim 14, wherein said binding partners are selected from the group consisting of antibodies, binding proteins, and nucleic acids.

24. The kit of claim 23, wherein said binding partners are nucleic acids.