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Generation and amplification of nucleic acids from ribonucleic acids

  • US 6,300,069 B1
  • Filed: 05/03/1999
  • Issued: 10/09/2001
  • Est. Priority Date: 05/03/1999
  • Status: Expired due to Term
First Claim
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1. In a method for generating a nucleic acid from an RNA template comprising the steps:

  • a) adding said RNA template to a reaction mixture, said reaction mixture comprising at least one reverse transcriptase and at least one nucleic acid polymerase, or derivatives thereof; and

    b) incubating said reaction mixture under conditions sufficient to allow polymerization of a nucleic acid molecule complementary to a portion of said RNA template, wherein the improvement comprises a reaction mixture further comprising a homopolymeric oligonucleotide at least 50 bases in length.

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