Sensor employing impedance measurements

• US 6,300,123 B1
• Filed: 07/19/1999
• Issued: 10/09/2001
• Est. Priority Date: 10/26/1996
• Status: Expired due to Term

• Current Assignee: Queen Mary And Westfield College
• Original Assignee: The Victoria University of Manchester
• Inventors: Warriner, Keith S. R., Vadgama, Pankaj M.
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US09/297,124
• Time in Patent Office: 813 Days
• Field of Search: 435/287.9, 435/817, 435/287.2, 435/287.1, 435/4, 435/26, 204/403, 422/82.01, 422/82.02, 436/537, 436/151, 436/149
• US Class Current: 435/287.1
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A sensor uses an immobilized affinity component capable of interacting with analyte species and being associated with a conducting polymer such that the interaction of the affinity component and the analyte induces change in the electrical properties of the polymer. An AC signal is applied to the polymer, and the induced change in impedance resulting from the interaction is measured. The impedance is measured at a frequency or frequencies corresponding to a peak or peaks in the relationship between frequency and impedance change for the polymer and the analyte. The measurement may be made by reference to the imaginary or real component of impedance. The polymer may be in the form of a layer bridging two electrodes between which the impedance is measured. The two electrodes may together define an interdigitated electrode assembly.

Claims

1. A sensor comprising:

an immobilised affinity component capable of interacting with a predetermined analyte species and being associated with a conducting polymer such that interaction of the affinity component and the analyte induces a change in electrical properties of the polymer,

means for applying an AC signal to the polymer, and

means for detecting the response of the polymer to the applied signal to detect the induced change,

wherein the detecting means comprises means for measuring the impedance of the polymer at a frequency selected to correspond to a peak in the relationship between frequency and impedance change for the polymer and the predetermined analyte.

2. A sensor as in claim 1, wherein the imaginary component of impedance is measured at a frequency at which there is a peak in the relationship between the imaginary component and frequency.

3. A sensor as in claim 1 wherein the real component of impedance is measured at a frequency at which there is a peak in the relationship between the real component and frequency.

4. A sensor as in claim 1 wherein the polymer is in the form of a layer bridging two electrodes between which the impedance is measured.

5. A sensor as in claim 4, wherein the two electrodes together define an interdigitated electrode assembly.

6. A sensor as in claim 4, wherein the polymer layer has a thickness of less than 5 μ

m.

7. A sensor as in claim 4, wherein the layer of the conducting polymer contains, or incorporates a layer of, immobilised affinity component.

8. A sensor as in claim 7, wherein the affinity component is entrapped within a gel layer provided on the conducting polymer layer on the side thereof remote from the electrodes and the gel thickness is less than 50 μ

m.

9. A sensor as in claim 1 wherein the affinity component is immobilised within the conducting polymer.

10. A sensor as in claim 1, wherein the affinity component is immobilised by entrapment in a gel provided on the conducting polymer.

11. A sensor as in claim 1, wherein the conducting polymer is poly(3-methylthiophene).

12. A sensor as in claim 1 wherein the affinity component is a macromolecule.

13. A sensor as in claim 1 wherein the affinity component is a biocomponent.

14. A sensor as in claim 13, wherein the biocomponent is an enzyme.

15. A sensor as in claim 14, wherein the enzyme is a kinase or dehydrogenase, or L(+) lactic dehydrogenase suitable for measuring pyruvate.

16. A sensor as in claim 15, wherein the conducting polymer is a poly(pyrrole).

17. A sensor as in claim 13, wherein the affinity component is an antibody, antigen, lectin or receptor.

18. A method for enhancing the sensitivity of a sensor said method comprising:

associating an immobilised affinity component capable of interacting with an analyte species with a conducting polymer such that the interaction of the affinity component and the analyte induces a change in the electrical properties of the polymer,

applying AC signals to the polymer, and

detecting a response of the polymer to the applied signals to detect the induced change,

wherein the impedance of the polymer is measured over a wide frequency range, at last one peak in the relationship between frequency and impedance change resulting from exposure of the polymer to the analyte is detected, and the sensor is operated at a frequency or frequencies corresponding to a detected peak or peaks.