Arrangement and method for performing chromatography

• US 6,303,029 B1
• Filed: 10/25/1999
• Issued: 10/16/2001
• Est. Priority Date: 10/25/1999
• Status: Expired due to Term

• Current Assignee: Indiana University Research & Technology Corporation (Indiana University)
• Original Assignee: ADVANCED RESEARCH & TECHNOLOGY INSTITUTE
• Inventors: Frost, Megan, Nurok, David
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US09/426,297
• Time in Patent Office: 722 Days
• Field of Search: 210/658, 210/198.3, 210/748, 210/243, 204/600, 436/162, 422/70
• US Class Current: 210/198.3
• CPC Class Codes: G01N 2030/285 electrically driven carrier
• CPC Class Codes: G01N 2030/3007 same temperature for whole column
• CPC Class Codes: G01N 2030/906 pressurised fluid phase
• CPC Class Codes: G01N 30/6047 with supporting means; Holders
• CPC Class Codes: G01N 30/90 Plate chromatography, e.g. thin layer or paper chromatography
• CPC Class Codes: G01N 30/92 Construction of the plate
• CPC Class Codes: G01N 30/94 Development

Abstract

An arrangement for performing chromatography with a mobile phase and a chromatographic bed having a stationary phase is provided. The arrangement includes a support assembly configured to support the stationary phase. The support assembly has a sealed cavity configured so that when the support assembly is supporting the stationary phase a portion of the chromatographic bed and the stationary phase extends into the sealed cavity. The arrangement further includes a bladder having a void defined therein. The bladder is positioned within the sealed cavity. The arrangement also includes a fluid positioned within the void of the bladder so that the bladder is urged toward the stationary phase within the sealed cavity. The arrangement further includes an anode positioned in contact with the mobile phase. The arrangement also includes a cathode positioned in contact with the stationary phase, wherein creating an electrical potential between the anode and the cathode causes the mobile phase to be advanced through the chromatographic bed positioned within the sealed cavity. An associated method is also provided.

Claims

1. An arrangement for performing chromatography with a mobile phase and a chromatographic bed having a stationary phase, comprising:

a support assembly configured to support said chromatographic bed and said stationary phase, said support assembly having a sealed cavity configured so that when said support assembly is supporting said stationary phase a portion of said chromatographic bed and said stationary phase extends into said sealed cavity;

a first electrode positioned in contact with said mobile phase; and

a second electrode positioned in contact with said stationary phase,

wherein creating an electrical potential between said first electrode and said second electrode causes said mobile phase to be advanced through said chromatographic bed positioned within said sealed cavity.

2. The arrangement of claim 1, wherein:

said first electrode includes an anode, and

said second electrode includes a cathode.

3. The arrangement of claim 2, wherein:

said stationary phase includes a thin layer chromatography plate having a cathode portion and an anode portion,

said mobile phase includes a liquid,

said anode portion of said thin layer chromatography plate is in contact with said liquid when said portion of said stationary phase is located within said sealed cavity,

said cathode is in contact with said cathode portion of said thin layer chromatography plate,

said anode is in contact with said liquid of said mobile phase, and

creating an electrical potential between said cathode and said anode causes said liquid of said mobile phase to migrate through said chromatographic bed in a direction away from said anode portion and toward said cathode portion.

4. The arrangement of claim 3, wherein:

said cathode includes a platinum wire attached to a piece of platinum foil, and

said platinum foil is positioned in contact with said cathode portion of said thin layer chromatography plate.

5. The arrangement of claim 1, further comprising:

a fluid positioned within said sealed cavity of said support assembly so that pressure within said sealed cavity is greater than pressure outside of said sealed cavity.

6. The arrangement of claim 1, further comprising:

a bladder having a void defined therein, said bladder being positioned within said sealed cavity; and

a fluid positioned within said void of said bladder so that said bladder is urged toward said stationary phase positioned within said sealed cavity.

7. The arrangement of claim 6, wherein:

said fluid positioned within said void of said bladder is a liquid.

8. The arrangement of claim 7, further comprising:

an absorbing element positioned in contact with said cathode portion of said thin layer chromatography plate so that said absorbing element absorbs said liquid when said electrical potential is created between said anode and said cathode.

9. The arrangement of claim 1, further comprising:

a housing secured to said support assembly, said housing having an interior chamber configured to receive a cooling fluid,

wherein said interior chamber is located relative to said stationary phase so that said cooling fluid is positioned in a heat exchange relationship with said stationary phase when said electrical potential is created between said anode and said cathode.

10. An arrangement for performing chromatography with a mobile phase and a chromatographic bed having a stationary phase, comprising:

a support assembly configured to support said stationary phase, said support assembly having a sealed cavity configured so that when said support assembly is supporting said stationary phase a portion of said chromatographic bed and said stationary phase extends into said sealed cavity;

a fluid positioned within said sealed cavity so that (i) said fluid is placed in a heat exchange relationship with said stationary phase and (ii) pressure within said sealed cavity is greater than pressure outside of said sealed cavity;

an anode positioned in contact with said mobile phase; and

a cathode positioned in contact with said stationary phase,

wherein creating an electrical potential between said anode and said cathode causes said mobile phase to be advanced through said chromatographic bed positioned within said sealed cavity.

11. The arrangement of claim 10, further comprising:

a pump in fluid communication with said sealed cavity,

wherein said pump advances said fluid through said sealed cavity via an entrance orifice defined in a first wall of said sealed cavity and an exit orifice defined in a second wall of said sealed cavity.

12. The arrangement of claim 10, wherein:

said stationary phase includes a thin layer chromatography plate having a cathode portion and an anode portion,

said mobile phase includes a liquid,

said anode portion of said thin layer chromatography plate is in contact with said liquid when said portion of said stationary phase is located within said sealed cavity,

said cathode is in contact with said cathode portion of said thin layer chromatography plate,

said anode is in contact with said liquid of said mobile phase, and

creating an electrical potential between said cathode and said anode causes said liquid of said mobile phase to migrate through said chromatographic bed in a direction away from said anode portion and toward said cathode portion.

13. The arrangement of claim 10, further comprising:

an absorbing element positioned in contact with said stationary phase so that said absorbing element absorbs said mobile phase when said electrical potential is created between said anode and said cathode.