System and method for emulating an in vivo environment of a tissue specimen

• US 6,303,286 B1
• Filed: 07/06/2000
• Issued: 10/16/2001
• Est. Priority Date: 12/07/1998
• Status: Expired due to Fees

• Current Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Original Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Inventors: Kosnik, Paul, Dennis, Robert G.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/611,241
• Time in Patent Office: 467 Days
• Field of Search: 435/1.1, 435/3, 435/173.1, 435/366, 435/375, 435/284.1, 435/286.1, 435/287.1, 435/289.1, 737/81, 737/88, 738/05, 364/506, 364/507, 364/508, 364/512, 364/514, 364/550, 364/551.01, 128/733, 128/740, 128/774
• US Class Current: 435/3
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli

Abstract

A system and method are provided for adaptively controlling a muscle tissue specimen in order to emulate its in vivo environment. The system includes at least one stimulator for stimulating the muscle tissue specimen based on an initial control signal, wherein the stimulation preferably includes electrical and/or mechanical stimulation. A response signal is generated based on a response of the muscle tissue specimen to the step of stimulating. The response signal preferably represents force production of the tissue specimen. A controller is provided for modifying the initial control signal based on the response signal to obtain a final control signal, wherein the final control signal is used to elicit a desired response from the muscle tissue specimen. Advantageously, the system and method of the present invention can be used to adaptively control the stimulation of a muscle tissue specimen in a tissue culture environment.

Claims

1. A method of emulating an in vivo environment of a muscle tissue specimen, the method comprising:

electrically stimulating the muscle tissue specimen based on an initial control signal;

generating a response signal corresponding to a response of the muscle tissue specimen to the step of stimulating; and

modifying the initial control signal based on the response signal to obtain a final control signal, wherein the final control signal is used to elicit a desired response from the muscle tissue specimen.

2. The method of claim 1, further including stimulating the muscle tissue specimen mechanically.

3. The method of claim 1, wherein the muscle tissue specimen includes skeletal muscle tissue.

4. The method of claim 1, wherein the response of the muscle tissue specimen comprises force production.

5. The method of claim 1, wherein the step of stimulating includes stimulating the muscle tissue specimen in culture.

6. The method of claim 1, further including stimulating the muscle tissue specimen chemically.

7. A method of emulating an in vivo environment of a tissue specimen, the method comprising:

electrically stimulating the tissue specimen based on an initial control signal;

generating a response signal corresponding to a response of the tissue specimen to the step of stimulating; and

modifying the initial control signal based on the response signal to obtain a final control signal, wherein the final control signal is used to elicit a desired response from the tissue specimen.

8. The method of claim 7, further including stimulating the tissue specimen mechanically.

9. The method of claim 7, further including stimulating the tissue specimen chemically.

10. The method of claim 7, wherein the tissue specimen includes muscle tissue.

11. The method of claim 7, wherein the tissue specimen includes nerve tissue.

12. The method of claim 7, wherein the step of stimulating includes stimulating the tissue specimen in culture.