Blue diode-pumped solid-state-laser based on ytterbium doped laser crystals operating on the resonance zero-phonon transition
First Claim
1. A ytterbium-doped solid state laser with an output wavelength in the ˜
- 490-496 nm region, comprising;
a resonant optical cavity;
a ytterbium-doped solid state laser gain medium within said resonant optical cavity;
means for optically pumping said laser gain medium;
an inter-cavity polarizer for forcing said solid state laser gain medium to generate laser light on the zero-phonon-line (ZPL) transition of the 2F5/2−
2F7/2 resonance emission band of said gain medium, wherein said inter-cavity polarizer is oriented to allow transmission of polarized light matching the predominant polarization of said ZPL transition of said ytterbium-doped solid state laser gain medium, while providing loss for non-ZPL transitions emitting predominantly in the orthogonal polarization with respect to the polarization of said ZPL of said ytterbium-doped solid state laser gain medium; and
a nonlinear harmonic doubler crystal for doubling the frequency of said zero-phonon-line (ZPL) transition of the 2F5/2−
2F7/2 resonance emission band of said gain medium.
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Abstract
The invention provides an efficient, compact means of generating blue laser light at a wavelength near ˜493+/−3 nm, based on the use of a laser diode-pumped Yb-doped laser crystal emitting on its zero phonon line (ZPL) resonance transition at a wavelength near ˜986+/−6 nm, whose fundamental infrared output radiation is harmonically doubled into the blue spectral region. The invention is applied to the excitation of biofluorescent dyes (in the ˜490-496 nm spectral region) utilized in flow cytometry, immunoassay, DNA sequencing, and other biofluorescence instruments. The preferred host crystals have strong ZPL fluorecence (laser) transitions lying in the spectral range from ˜980 to ˜992 nm (so that when frequency-doubled, they produce output radiation in the spectral range from 490 to 496 nm). Alternate preferred Yb doped tungstate crystals, such as Yb:KY(WO4)2, may be configured to lase on the resonant ZPL transition near 981 nm (in lieu of the normal 1025 nm transition). The laser light is then doubled in the blue at 490.5 nm.
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Citations
42 Claims
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1. A ytterbium-doped solid state laser with an output wavelength in the ˜
- 490-496 nm region, comprising;
a resonant optical cavity;
a ytterbium-doped solid state laser gain medium within said resonant optical cavity;
means for optically pumping said laser gain medium;
an inter-cavity polarizer for forcing said solid state laser gain medium to generate laser light on the zero-phonon-line (ZPL) transition of the 2F5/2−
2F7/2 resonance emission band of said gain medium, wherein said inter-cavity polarizer is oriented to allow transmission of polarized light matching the predominant polarization of said ZPL transition of said ytterbium-doped solid state laser gain medium, while providing loss for non-ZPL transitions emitting predominantly in the orthogonal polarization with respect to the polarization of said ZPL of said ytterbium-doped solid state laser gain medium; and
a nonlinear harmonic doubler crystal for doubling the frequency of said zero-phonon-line (ZPL) transition of the 2F5/2−
2F7/2 resonance emission band of said gain medium.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
- 490-496 nm region, comprising;
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20. A method for optically pumping a dye solution, comprising:
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providing a ytterbium-doped solid state laser gain medium within an optical cavity;
optically pumping said laser gain medium;
forcing said solid state laser gain medium to emit laser light on the zero-phonon-line (ZPL) transition of the 2F5/2−
2F7/2 resonance emission band of said gain medium, wherein said step of forcing said solid state laser gain medium to emit laser light on the zero-phonon-line (ZPL) transition is carried out with an inter-cavity polarizer, wherein said inter-cavity polarizer is oriented to allow transmission of polarized light matching the predominant polarization of said ZPL transition of said ytterbium-doped solid state laser gain medium, while increasing the effective losses for nonZPL transitions emitting predominantly in the orthogonal polarization with respect to the polarization of said ZPL of said ytterbium-doped solid state laser gain medium;
doubling the frequency of said zero-phonon-line (ZPL) transition of said 2F5/2−
2F7/2 resonance emission band of said gain medium to produce second harmonic light within the wavelength range of 490 nm to 496 nm; and
directing said second harmonic light onto a dye solution having an excitation band within the wavelength range of 490 nm to 496 nm. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
selectively labeling the bases of DNA fragments with dye selected from a group consisting of fluorescein isothiocyanate dye and phycoerthrin dye to produce dye labeled DNA fragments;
passing said dye labeled DNA fragments through multiple channels of a multichannel electrophoresis sequencing apparatus;
illuminating said muliple channels with said second harmonic light, wherein one fluorescent color will fluoresce for each of the labeled bases of DNA; and
detecting the position of each said fluorescent color to determine the base sequence of said DNA fragments.
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23. The method of claim 22, wherein the step of forcing said solid state laser gain medium to emit laser light on the zero-phonon-line (ZPL) transition includes forming said optical cavity with dichroic optics having a reflection bandwidth narrow enough to allow laser oscillation only on said zero-phonon-line (ZPL) transition.
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24. The method of claim 22, wherein the step of producing laser radiation includes providing an ytterbium-doped solid state laser gain medium comprising a crystal having an apatite structure.
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25. The method of claim 22, wherein the step of producing laser radiation includes providing an ytterbium-doped solid state laser gain medium comprising a crystal having an apatite structure, doped with trivalent ytterbium (Yb3+).
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26. The method of claim 22, wherein the step of producing laser radiation includes providing a ytterbium-doped solid state laser gain medium comprising a crystal having an apatite structure, doped with trivalent ytterbium (Yb3+), wherein said laser gain medium is selected from a group consisting of C-FAP, S-FAP, S-VAP, B-FAP and M5(AO4)3F where A is selected from a group consisting of P and V and M is selected from a group consisting of Ca, Sr, Br and Pb.
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27. The method of claim 22, wherein the step of producing laser radiation includes providing a ytterbium-doped solid state laser gain medium comprising a double tungstate crystal of the form (Ha)(RE)(WO4)2, doped with trivalent ytterbium (Yb3+), and wherein Ha is a monovalent alkali ion selected from a group consisting of Li, Cs, Na, Rb and K, and wherein RE is a trivalent rare earth ion selected from a group consisting of La, Gd and Lu ions.
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28. The method of claim 22, wherein the step of producing laser radiation includes providing a ytterbium-doped solid state laser gain medium comprises a crystal selected from a group consisting of a C-FAP crystal and an S-FAP crystal, wherein said crystal has an apatite structure that is doped with trivalent ytterbium (Yb3+).
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29. The method of claim 20, further comprising:
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selectively staining chromosomal DNA fragments with fluorescent dye selected from a group consisting of fluorescein isothiocyanate dye and phycoerthrin dye to produce dye stained chromosomal DNA fragments;
passing said dye stained chromosomal DNA fragments through multiple channels of a multi-channel electrophoresis sequencing apparatus, wherein said second harmonic light is directed onto said dye solution by illuminating said muliple channels with said second harmonic light, wherein one fluorescent color will fluoresce for each of the four genetic letters A, G, T, C of said chromosomal DNA; and
detecting the position of each said fluorescent color to read the genetic letter sequence of said chromosomal DNA fragments.
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30. The method of claim 29, wherein the step of forcing said solid state laser gain medium to emit laser light on the zero-phonon-line (ZPL) transition includes forming said optical cavity with dichroic optics having a reflection bandwidth narrow enough to allow laser oscillation only on said zero-phonon-line (ZPL) transition.
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31. The method of claim 29, wherein the step of producing laser radiation includes providing an ytterbium-doped solid state laser gain medium comprising a crystal having an apatite structure.
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32. The method of claim 29, wherein the step of producing laser radiation includes providing an ytterbium-doped solid state laser gain medium comprising a crystal having an apatite structure, doped with trivalent ytterbium (Yb3+).
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33. The method of claim 29, wherein the step of producing laser radiation includes providing a ytterbium-doped solid state laser gain medium comprising a crystal having an apatite structure, doped with trivalent ytterbium (Yb3+), wherein said laser gain medium is selected from a group consisting of C-FAP, S-FAP, S-VAP, B-FAP and M5(AO4)3F where A is selected from a group consisting of P and V and M is selected from a group consisting of Ca, Sr, Br and Pb.
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34. The method of claim 29, wherein the step of producing laser radiation includes providing a ytterbium-doped solid state laser gain medium comprising a double tungstate crystal of the form (Ha)(RE)(WO4)2, doped with trivalent ytterbium (Yb3+), and wherein Ha is a monovalent alkali ion selected from a group consisting of Li, Cs, Na and K, and wherein RE is a trivalent rare earth ion selected from a group consisting of La, Gd and Lu ions.
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35. The method of claim 29, wherein the step of producing laser radiation includes providing a ytterbium-doped solid state laser gain medium comprises a C-FAP crystal having an apatite structure that is doped with trivalent ytterbium (Yb3+).
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36. The method of claim 20, further comprising:
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selectively staining blood or bone marrow cells with antibodies tagged with at least one fluorescent dye, wherein said at least one fluorescent dye is selected from a group consisting of fluorescein isothiocyanate dye and phycoerthrin dye to produce dye stained cells;
passing said dye stained cells in liquid suspension single file in a cell flow stream through a flow cytometer apparatus, wherein said second harmonic light is directed onto said dye solution by illuminating said cell flow stream with said second harmonic light, wherein each fluorescent dye excited will fluoresce at its characteristic wavelength; and
detecting said characteristic wavelength to immunotype cells labeled with specific antibodies.
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37. The method of claim 36, wherein the step of forcing said solid state laser gain medium to emit laser light on the zero-phonon-line (ZPL) transition includes forming said optical cavity with dichroic optics having a reflection bandwidth narrow enough to allow laser oscillation only on said zero-phonon-line (ZPL) transition.
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38. The method of claim 36, wherein the step of producing laser radiation includes providing an ytterbium-doped solid state laser gain medium comprising a crystal having an apatite structure.
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39. The method of claim 36, wherein the step of producing laser radiation includes providing an ytterbium-doped solid state laser gain medium comprising a crystal having an apatite structure, doped with trivalent ytterbium (Yb3+).
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40. The method of claim 36, wherein the step of producing laser radiation includes providing a ytterbium-doped solid state laser gain medium comprising a crystal having an apatite structure, doped with trivalent ytterbium (Yb3+), wherein said laser gain medium is selected from a group consisting of C-FAP, S-FAP, B-FAP, S-VAP and M5(AO4)3F where A is selected from a group consisting of P and V and M is selected from a group consisting of Ca, Sr, Br and Pb.
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41. The method of claim 36, wherein the step of producing laser radiation includes providing a ytterbium-doped solid state laser gain medium comprising a double tungstate crystal of the form (Ha)(RE)(WO4)2, doped with trivalent ytterbium (Yb3+), and wherein Ha is a monovalent alkali ion selected from a group consisting of Li, Cs, Na, Rb and K, and wherein RE is a trivalent rare earth ion selected from a group consisting of La, Gd and Lu ions.
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42. The method of claim 36, wherein the step of producing laser radiation includes providing a ytterbium-doped solid state laser gain medium comprises a C-FAP crystal having an apatite structure that is doped with trivalent ytterbium (Yb3+).
Specification