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Methods and compositions for conducting processes in microfluidic devices

  • US 6,306,273 B1
  • Filed: 04/13/1999
  • Issued: 10/23/2001
  • Est. Priority Date: 04/13/1999
  • Status: Expired due to Term
First Claim
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1. A method for transporting a liquid containing at least two chemical components in a selected direction in a channel of a microfluidic system, by electrokinesis, wherein said at least two chemical components include a protein having one charge at a selected buffer pH and another component having the opposite charge at the same pH, said method comprising(a) selecting a microfluidic system having a channel formed of an acrylic-based polymer;

  • (b) exposing the walls of the channel to a solution containing 0.0015% to 5% weight percent of a charged hydrophilic amine polymer, wherein charges on said charged hydrophilic polymer are randomly distributed;

    (c) after said exposing, filling the channel with a solution containing the at least two chemical components at the selected pH; and

    (d) applying a voltage from one point along said channel to a different point along said channel whereby the differently charged chemical components are transported in said selected direction along said channel.

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