Culture dish

• US 6,306,646 B1
• Filed: 10/18/2000
• Issued: 10/23/2001
• Est. Priority Date: 03/03/1998
• Status: Expired due to Term

• Current Assignee: Weidmann Plastics Technology AG (Techniplas LLC)
• Original Assignee: Weidmann Plastics Technology AG (Techniplas LLC)
• Inventors: Saad, Bashar, Callenbach, Tilo
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/623,398
• Time in Patent Office: 370 Days
• Field of Search: 422/102, 435/288.3, 435/288.4, 435/288.5, 435/305.1, 435/305.2, 435/305.3
• US Class Current: 435/305.1
• CPC Class Codes: C12M 23/00 Constructional details, e.g. recesses, hinges flow directing inserts in C12M27/18-C12M27/24; apparatus for chemical or physical processes in general B01J, chemical or physical laboratory apparatus in general B01L
• CPC Class Codes: C12M 23/20 Material Coatings immunocoatings C12M25/00
• CPC Class Codes: C12N 2535/10 Patterned coating
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

The invention relates to a culture dish consisting of a plastic material and intended for growing cell and tissue cultures (6). The growth surface (5) has a micro-structure and raised areas (9) whose height (A) is less than 110 micrometres and preferably less than approximately 100 micrometres. The micro-structure (8) of the growth surface increases the cell yield, with adherent cells being both optically easily accessible and readily harvested.

Claims

1. A culture vessel made of plastic for growing cell and tissue cultures (6), with a growing surface that is provided with a microstructure (5) having elevations (9), whose height (A) is less than 110 micrometers, and indentations, whose bottom surface is a flat surface (12), characterized in that the elevations (9) each have an upper flat surface (10), and that said flat surfaces (12) are arranged between the elevations (9), and that adjacent indentations are connected with one another, wherein the elevations (9) are truncated pyramids or cones.

2. Culture vessel as claimed in claim 1, characterized in that the height (A) is less than 100 micrometers and preferably 10 to 60 micrometers.

3. Culture vessel as claimed in claim 1, characterized in that the flat surface (10) has a width (B) that is smaller than 300 micrometers.

4. A culture vessel as claimed in claim 2, wherein the flat surfaces (12) between the elevations (9) each have a width (C) or length that is smaller than 300 micrometers.

5. A culture vessel as claimed in claim 1, wherein the elevations (9) each have several lateral surfaces (11) which are inclined in relation to or extend perpendicularly from the flat surfaces (12) and are a part of said growing surface (5).

6. Culture vessel as claimed in claim 1, wherein it is produced from a thermoplastic by means of an injection molding process.

7. Culture vessel as claimed in claim 1, wherein it is made from an amorphous thermoplastic.

8. Culture vessel as claimed in claim 7, characterized in that the thermoplastic is polystyrene.

9. Culture vessel as claimed in claim 1, wherein it is surface treated for optimal cell adhesion and cell growth, for example, in a microwave plasma.

10. Culture vessel as claimed in claim 1, wherein the growing surface (5) has regularly arranged elevations (9) and these elevations (9) have geometric and defined surfaces (10, 11, 12).

11. Culture vessel as claimed in claim 1, wherein all or a substantial part of the elevations (9) are identical in shape.

12. A culture vessel as claimed in claim 1, wherein the growing surface (5) includes said flat surfaces (12), wherein said flat surfaces (12) are identical and regularly arranged between the elevations (9).

13. A culture vessel as claimed in claim 12, characterized in that said flat surfaces (12) between the elevations (9) are flat and rectangular.